Reinhart W H, Sung L P, Sung K L, Bernstein S E, Chien S
Department of Physiology, Columbia University, College of Physicians and Surgeons, New York City, New York.
Am J Hematol. 1988 Dec;29(4):195-200. doi: 10.1002/ajh.2830290404.
The membrane skeleton of the red blood cell plays an important role in the determination of cell deformability and cell shape. Under various in vitro conditions, red blood cells undergo an echinocytic or stomatocytic shape transformation. The mechanism of this fundamental process is not well understood. We have studied the red cell shape transformation in normoblastic anemia mice (nb/nb) and spherocytic anemia mice (sph/sph), which are deficient in ankyrin and spectrin, respectively. We found that both ankyrin-deficient cells (nb/nb) and spectrin-deficient cells (sph/sph) have a reduced capacity to undergo echinocytic transformation with various echinocytogenic treatments, that is, incubation with sodium salicylate (40 and 120 mM), calcium loading (50 microM A23187 + 2.2 mM Ca2+), or metabolic depletion (24 hr at 37 degrees C). These results suggest that the functional integrity of the membrane skeleton is essential for the maintenance and transformation of the red cell shape.
红细胞的膜骨架在决定细胞变形性和细胞形状方面起着重要作用。在各种体外条件下，红细胞会发生棘状红细胞或口形红细胞形状转变。这一基本过程的机制尚未完全了解。我们研究了分别缺乏锚蛋白和血影蛋白的正成红细胞性贫血小鼠（nb/nb）和球形红细胞性贫血小鼠（sph/sph）的红细胞形状转变。我们发现，无论是缺乏锚蛋白的细胞（nb/nb）还是缺乏血影蛋白的细胞（sph/sph），在接受各种致棘状红细胞生成处理时，即与水杨酸钠（40和120 mM）孵育、钙负载（50 microM A23187 + 2.2 mM Ca2+）或代谢耗竭（37摄氏度下24小时），发生棘状红细胞转变的能力均降低。这些结果表明，膜骨架的功能完整性对于维持和转变红细胞形状至关重要。
