[Effect of applying phosphorus bacteria fertilizer on bacterial diversity and phosphorus availability in reclaimed soil.].

A located field experiment of applying phosphorus bacteria fertilizer for five years was carried out to study the effects of applying phosphorus bacteria fertilizer on the characteristics of microbial community structure in reclaimed soil. We studied the diversity of bacterial community using 16S rDNA gene sequencing and analyzed the relationship between bacterial community and Olsen-P, alkaline phosphatase. Seven treatments including control, chemical fertilizer, manure, manure and chemical fertilizer, chemical fertilizer and phosphorus bacteria, manure and phosphorus bacteria, and, manure,chemical fertilizer and phosphorus bacteria were conducted. The results showed that the relative abundance of Actinobacteria and Proteobacteria in reclaimed soil was the largest, which was 21.6％-32.2％ and 13.8％-28.9％, respectively. Operational taxonomic units (OTU) number and Chao1 index of the treatment of manure, chemical fertilizer and phosphorus bacteria fertilizer was 809 and 26190, which was the highest. Phosphorus bacteria fertilizer could improve the relative abundance of soil Actinomycetes and Proteobacteria and decrease that of soil Acidobacteria, Thermotogae and Nitrospira, and had stimulatory effect on Nocardioides and Flexibacter. The treatment of manure, chemical fertilizer and phosphorus bacteria fertilizer could improve the Olsen-P and alkaline phosphatase activities in reclaimed soil. Correlation coefficients between Proteobacteria and Olsen-P, alkaline phosphatase were the highest (0.900 and 0.955). To a certain extent, Proteobacteria could be used as the sensitivity index of soil phosphorus availability.