Hunan Soil and Fertilizer Institute, Changsha, 410125, People's Republic of China.
Sci Rep. 2020 Aug 11;10(1):13540. doi: 10.1038/s41598-020-70612-y.
The soil physicochemical properties and soil microbial communities were affected by different fertilizer management. Fertilizer regime were closely relative to the soil texture and nutrient status in a double-cropping paddy field of southern China. However, there was limited information about the influence of different manure nitrogen (N) input on soil microbial communities in a double-cropping rice (Oryza sativa L.) field. Therefore, the short-term different manure N input rate management on soil bacterial and fungal diversity in a double-cropping paddy field of southern China were studied by using Illumina sequencing and quantitative real-time polymerase chain reaction technology in the present paper. The filed experiment were including 100% N of chemical fertilizer (M0), 30% N of organic manure and 70% N of chemical fertilizer (M30), 50% N of organic manure and 50% N of chemical fertilizer (M50), 100% N of organic manure (M100), and without N fertilizer input as control (CK). The results showed that diversity indices of soil microbial communities with application of organic manure and chemical N fertilizer treatments were higher than that of CK treatment. Application of organic manure and chemical N fertilizer management increase soil bacterial abundance of the phylum Actinobacteria, Proteobacteria and Gammaproteobacteria, and soil fungi abundance of the phylum Basidiomycota and Zygomycota were also increased. Compared with CK treatment, the value of Richness, Shannon and McIntosh indices, and taxonomic diversity were increased with M30, M50 and M100 treatments. This finding demonstrated that M30, M50 and M100 treatments modify soil bacterial and fungal diversity. Therefore, the combined application of organic manure and chemical fertilizer N management could significantly increase the abundance of profitable functional bacteria and fungi species in a double-cropping rice field of southern China.
不同的肥料管理会影响土壤理化性质和土壤微生物群落。肥料制度与中国南方双季稻田的土壤质地和养分状况密切相关。然而，关于不同有机肥氮（N）输入对双季稻田土壤微生物群落的影响，信息有限。因此，本文采用 Illumina 测序和实时定量聚合酶链反应技术，研究了中国南方双季稻田短期不同有机肥 N 投入率管理对土壤细菌和真菌多样性的影响。田间试验包括 100%化肥 N（M0）、30%有机肥和 70%化肥 N（M30）、50%有机肥和 50%化肥 N（M50）、100%有机肥（M100）和不施氮肥作为对照（CK）。结果表明，施用有机肥和化肥 N 处理的土壤微生物群落多样性指数均高于 CK 处理。施用有机肥和化肥管理增加了土壤放线菌门、变形菌门和γ-变形菌门的细菌丰度，也增加了土壤担子菌门和接合菌门的真菌丰度。与 CK 处理相比，M30、M50 和 M100 处理的丰富度、香农和麦克因托什指数以及分类多样性值增加。这一发现表明，M30、M50 和 M100 处理可改变土壤细菌和真菌多样性。因此，有机肥和化肥 N 的联合施用可显著增加中国南方双季稻田中有益功能细菌和真菌的丰度。
Zotero 插件
