Xu Dong Mei, Rao Gui Wei
College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China.
Ying Yong Sheng Tai Xue Bao. 2016 Sep;27(9):3029-3034. doi: 10.13287/j.1001-9332.201609.024.
In order to reveal the toxicity effect and risk of co-exposure of Cu and chlorpyrifos in the current soil environment of China, using the earthworm Eisenia fetida as model organism, the effect of single, and co-exposure of Cu and chlorpyrifos on the growth and antioxidant response of earthworm were analyzed by using the methods of filter paper test and artificial soil test. The results showed that the growth rate of earthworm tended to decrease with the increase of single exposure concentrations of Cu and chlorpyrifos in artificial soil test. The mass of earthworm had a relatively flat changing rate with the co-exposure of Cu and chlorpyrifos. Using the addition of probability, the q values of different concentration groups of Cu and chlorpyrifos on the earthworm mass were less than 0.55, which indicated significant antagonistic effect. The joint effect of Cu and chlorpyrifos on the activities of CAT and SOD indicated antagonistic effect in filter paper test, while the effects of co-exposure of Cu and chlorpyrifos on the contents of GSH and MDA showed a synergy effect of low concentrations and antagonism effect of high concentrations.
为揭示在中国当前土壤环境中铜与毒死蜱共同暴露的毒性效应及风险，以蚯蚓 Eisenia fetida 为模式生物，采用滤纸试验和人工土壤试验方法，分析了铜与毒死蜱单一及共同暴露对蚯蚓生长和抗氧化反应的影响。结果表明，在人工土壤试验中，随着铜和毒死蜱单一暴露浓度的增加，蚯蚓的生长速率呈下降趋势。铜与毒死蜱共同暴露时，蚯蚓的质量变化率相对平稳。采用相加概率法，不同浓度组的铜和毒死蜱对蚯蚓质量的 q 值均小于 0.55，表明存在显著拮抗作用。在滤纸试验中，铜与毒死蜱对 CAT 和 SOD 活性的联合作用表现为拮抗作用，而铜与毒死蜱共同暴露对 GSH 和 MDA 含量的影响则表现为低浓度协同作用和高浓度拮抗作用。
