School of Chemical Engineering, Sungkyunkwan University, Republic of Korea.
School of Pharmacy, Sungkyunkwan University, 16419 Suwon, Republic of Korea.
Biosens Bioelectron. 2018 Aug 15;113:82-87. doi: 10.1016/j.bios.2018.04.061. Epub 2018 May 1.
Amyloid-beta oligomers (AβΟ) are considered to be reliable biomarkers for the diagnosis of Alzheimer' disease (AD), and the cellular prion protein (PrP) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrP receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The developed PrPc/PPyCOOH biosensor exhibited extremely low detection limit of 10 pM with high sensitivity which is desirable for the early diagnosis of AD. The sensing performance was further confirmed by the mice infected with AD. The proposed sensor emerged as a promising tool for the early detection of AβO.
淀粉样蛋白-β寡聚物（AβΟ）被认为是阿尔茨海默病（AD）诊断的可靠生物标志物，细胞朊蛋白（PrP）被鉴定为 AβΟ的受体。我们使用导电聚（吡咯-2-羧酸）（PPyCOOH）连接剂和 PrP 受体，展示了一种无需标记和抗体的电化学生物传感器，用于选择性检测 AβΟ。在生物传感器的制备过程中，每个步骤均通过电化学阻抗谱和循环伏安法进行了表征。开发的 PrPc/PPyCOOH 生物传感器具有非常低的检测限 10 pM 和高灵敏度，非常适合 AD 的早期诊断。通过感染 AD 的小鼠进一步证实了传感性能。该传感器有望成为早期检测 AβΟ 的有力工具。
