冷却时猪髂内皮细胞内冰晶形成的低温显微镜分析。
Cryomicroscopic analysis of intracellular ice formation in porcine iliac endothelial cells upon cooling.
作者信息
Li Y, Panhwa F, Chen Z, Yuan F, Ji X, Hu P, Zhao G
机构信息
Centre for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China; Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs, Hefei, China.
Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei, China.
出版信息
Cryo Letters. 2017 Jul/Aug;38(4):315-320.
UNLABELLED
BACKGROUND: Intracellular ice formation (IIF) plays an important role in cryopreservation and cryosurgery. IIF in porcine iliac endothelial cells (PIECs) has not been fully investigated.
OBJECTIVE
To analyze the phenomenon of IIF in PIECs during freezing.
MATERIALS AND METHODS
The cryomicroscopy system was used for observation of the cell morphology and for the count of IIF during freezing, while the theoretical model for probability IIF (PIF) was used to determine the nucleation kinetic and thermodynamic parameters.
RESULTS
PIF was observed at 40, 80 and 100 degree C/min upon cooling and 100 degree C/min upon warming, in the presence and absence of 1 M dimethyl sulfoxide (Me2SO). The kinetic and thermodynamic parameters were obtained by fitting the PIF model to experimental data.
CONCLUSION
PIF increases along with the increased cooling rate. The addition of 1 M dimethyl sulfoxide (Me2SO) decreases the onset IIF temperature, but increases PIF. Cooling rate and Me2SO concentration significantly affect IIF. The finding has significant implications in both cryopreservation and cryosurgery.
未标记
背景：细胞内冰晶形成（IIF）在冷冻保存和冷冻手术中起重要作用。猪髂内皮细胞（PIECs）中的IIF尚未得到充分研究。
目的
分析冷冻过程中PIECs内的IIF现象。
材料与方法
冷冻显微镜系统用于观察细胞形态和冷冻过程中IIF的计数，而概率IIF（PIF）的理论模型用于确定成核动力学和热力学参数。
结果
在有和没有1 M二甲基亚砜（Me2SO）的情况下，冷却时在40、80和100℃/分钟以及升温时在100℃/分钟观察到PIF。通过将PIF模型拟合到实验数据获得动力学和热力学参数。
结论
PIF随着冷却速率的增加而增加。添加1 M二甲基亚砜（Me2SO）会降低IIF起始温度，但会增加PIF。冷却速率和Me2SO浓度显著影响IIF。这一发现对冷冻保存和冷冻手术都具有重要意义。
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