State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
ARC Industrial Transformation Training Centre for Functional Grains, Wagga Wagga, NSW 2678, Australia.
Food Res Int. 2018 Jun;108:1-7. doi: 10.1016/j.foodres.2018.02.074. Epub 2018 Mar 1.
In this work, a double shell material chitosan (CS)-recombinant soybean seed H-2 ferritin (H-2F) was fabricated to encapsulate epigallocatechin gallate (EGCG) molecules. EGCG-loaded H-2F complex (EHF) was firstly prepared by taking advantages of the reversible self-assembly of the ferritin, and the EHF-CS composite (EHFC) was fabricated by electrostatic interactions with binding number n of (4.1 ± 0.11) and binding constant K of ((5.3 ± 0.2) × 10 M), respectively. It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69% (w/w). SDS-PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract. In addition, the chitosan-ferritin double shells were beneficial for the transport of EGCG across the Caco-2 monolayer model based on ferritin uptake. This work demonstrates a novel method to promote stabilization and absorption of food bioactive molecules.
在这项工作中，构建了一种双层壳材料壳聚糖（CS）-重组大豆种子 H-2 铁蛋白（H-2F），以包裹表没食子儿茶素没食子酸酯（EGCG）分子。首先利用铁蛋白的可逆自组装优势制备了 EGCG 负载的 H-2F 复合物（EHF），然后通过静电相互作用制备了 EHF-CS 复合物（EHFC），结合数 n 为（4.1±0.11），结合常数 K 为（（5.3±0.2）×10⁻⁴M）。计算表明，约有 12.6 个 EGCG 分子可以被封装在一个 H-2F 铁蛋白笼中，包封效率为 9.69%（w/w）。SDS-PAGE 分析表明，CS 与 H-2F 的结合可以抑制胃蛋白酶和胰蛋白酶对铁蛋白的降解；EHFC 中 EGCG 分子的稳定性也在模拟胃肠道中得到显著提高。此外，基于铁蛋白摄取，壳聚糖-铁蛋白双层壳有利于 EGCG 穿过 Caco-2 单层模型的转运。这项工作展示了一种促进食品生物活性分子稳定和吸收的新方法。
