Li Ying, Wang Ziyin, Xia Weirong, Chai Zhi, Feng Jin, Teng Cong, Ma Kaiyang, Hu Xindi, Xu Lujing
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014 People's Republic of China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013 People's Republic of China.
Food Sci Biotechnol. 2025 Feb 3;34(9):1867-1878. doi: 10.1007/s10068-025-01826-x. eCollection 2025 May.
The application of Ala-His-Leu-Leu (AHLL), an angiotensin converting enzyme (ACE) inhibitory peptide, is limited due to its low stability. In this work, horse spleen apoferritin (HSF) deposited with a layer of sodium alginate (SA) was employed to carry AHLL. The HSF encapsulation and SA decoration exhibited remarkable protection capacity for loaded AHLL from UV irradiation and thermal treatment. The ACE inhibitory activity of AHLL-HSF@SA nanoparticles maintained 60%-75% within the digestion process since the compact structure and the coverage of enzyme reaction sites. Intestinal permeability investigation unveiled that HSF encapsulation facilitated the AHLL absorption, and the value was (2.70 ± 0.02) × 10 cm/s. Moreover, the transepithelial transport of free AHLL was primarily mediated through the paracellular pathway, whereas the transport of AHLL-HSF nanoparticles might rely on AP2-mediated endocytosis. Owing to positive effects on structural stability, release property and intestinal absorption, polysaccharides coated ferritin is a promising vehicle for peptides.
The online version contains supplementary material available at 10.1007/s10068-025-01826-x.
血管紧张素转换酶（ACE）抑制肽Ala-His-Leu-Leu（AHLL）由于稳定性低，应用受到限制。在这项工作中，用一层海藻酸钠（SA）包被的马脾脱铁铁蛋白（HSF）来运载AHLL。HSF包封和SA修饰对负载的AHLL表现出显著的保护能力，使其免受紫外线照射和热处理的影响。由于结构紧凑以及酶反应位点被覆盖，AHLL-HSF@SA纳米颗粒在消化过程中的ACE抑制活性保持在60%-75%。肠道通透性研究表明，HSF包封促进了AHLL的吸收，其值为(2.70±0.02)×10 cm/s。此外，游离AHLL的跨上皮转运主要通过细胞旁途径介导，而AHLL-HSF纳米颗粒的转运可能依赖于AP2介导的内吞作用。由于对结构稳定性、释放特性和肠道吸收有积极影响，多糖包被的铁蛋白是一种很有前途的肽载体。
在线版本包含可在10.1007/s10068-025-01826-x获取的补充材料。
