State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
Department of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang, Henan, China.
Food Res Int. 2018 Jun;108:161-171. doi: 10.1016/j.foodres.2018.03.035. Epub 2018 Mar 13.
Proteins and polysaccharides have been widely used as wall materials to prepare novel carriers for the encapsulation of a single bioactive component or co-encapsulation of different bioactive components. Emulsion-filled gel beads generally contain three essential components: oil droplets, interface membrane of emulsifier, and gel matrix. In this study, emulsion-filled gel beads were prepared by the combination of heat-denatured whey protein isolate (WPI)'s emulsification and alginate's gelation at various protein and oil contents, in order to discuss simultaneous encapsulation, protection and release of hydrophobic α-tocopherol and amphiphilic resveratrol. High efficiency for the co-encapsulation of resveratrol and α-tocopherol was achieved for the emulsion-filled gel beads containing 1% WPI and 1%-4% oil. WPI content was more important than the content of oil for the protection of resveratrol while the content of oil was more important for the encapsulation and protection of α-tocopherol during storage. The release of α-tocopherol was <20% while resveratrol was rapidly released during digestion. Resveratrol remained 73% after released from the emulsion-filled gel beads containing 1% WPI and 1%-2% oil for 6 h. These results should be useful for the development of biopolymer-based carriers for the delivery of different bioactive components.
蛋白质和多糖已被广泛用作壁材，用于制备封装单一生物活性成分或共封装不同生物活性成分的新型载体。乳液填充凝胶珠通常包含三个必需成分：油滴、乳化剂的界面膜和凝胶基质。在本研究中，通过乳状液中热变性乳清分离蛋白（WPI）的乳化和藻酸盐凝胶化的组合，在不同蛋白质和油含量下制备了乳液填充凝胶珠，以讨论疏水性α-生育酚和两亲性白藜芦醇的同时封装、保护和释放。对于含有 1% WPI 和 1%-4%油的乳液填充凝胶珠，实现了白藜芦醇和 α-生育酚的高效共封装。对于白藜芦醇的保护，WPI 含量比油含量更重要，而对于 α-生育酚的封装和保护，油含量在储存过程中更为重要。α-生育酚的释放量<20%，而白藜芦醇在消化过程中迅速释放。在含有 1% WPI 和 1%-2%油的乳液填充凝胶珠中释放 6 小时后，白藜芦醇仍保留 73%。这些结果对于开发基于生物聚合物的不同生物活性成分传递载体应该是有用的。
