[调整血小板聚集试验的血小板计数]

[Adjusting Platelet Counts for Platelet Aggregation Tests].

作者信息

Ling Li-Qin, Yang Xin-Chun, Chen Hao, Liu Chao-Nan, Chen Si, Jiang Hong, Jin Ya-Xiong, Zhou Jing

机构信息

Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China.

Faculty of Laboratory Medicine,West China School of Medicine/West China Hospital, Chengdu 610041,China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 Mar;49(2):276-279.

PMID:29737075

Abstract

OBJECTIVE

To explore a better method to adjust platelet counts for light transmission aggregometry (LTA).

METHODS

Blood samples from 36 healthy participants aged from 18 to 50 yr. were collected.Platelet-rich plasma (PRP) was diluted using platelet-poor plasma (PPP) and physiological saline (PS),respectively,in a ratio of 1.5,2,2.5 and 3 times. Platelet aggregation was induced by adenosine diphosphate (ADP),arachidonic acid (ARA),collagen (COL), epinephrine (EPI),or ristocetin (RIS). The maximal aggregation rates (MAs) of different approaches were compared. We also compared the MAs induced by RIS between PRP-obtained-PPP and whole blood-obtained-PPP (2 100× 5 min).

RESULTS

Compared with the original PRP,the MAs induced by ADP,ARA,and EPI decreased in PPP-adjusted PRP (significant at 2-3 times dilution ratio,<0.05),but not in PS-adjusted PRP (>0.05). The MA induced by RIS decreased in PS-adjusted PRP (significant at all dilution ratios,<0.05),but not in PPP-adjusted PRP (>0.05). No changes in the MA induced by COL were found in PS-adjusted PRP and PPP-adjusted PRP (>0.05). Whole blood-obtained-PPP (2 100× 5 min) had the same MA induced by ristocetin compared with PRP-obtained-PPP (>0.05).

CONCLUSION

PS is recommended for adjusting platelets counts for platelet aggregation induced by ADP,ARA,COL and EPI. Whole blood-obtained-PPP (2 100 × 5 min) is recommended for RIS-induced aggregation as a matter of convenience.

摘要

目的

探索一种更好的方法来调整用于光透射聚集法（LTA）的血小板计数。

方法

采集36名年龄在18至50岁之间的健康参与者的血样。分别使用乏血小板血浆（PPP）和生理盐水（PS）以1.5、2、2.5和3倍的比例稀释富血小板血浆（PRP）。用二磷酸腺苷（ADP）、花生四烯酸（ARA）、胶原（COL）、肾上腺素（EPI）或瑞斯托霉素（RIS）诱导血小板聚集。比较不同方法的最大聚集率（MAs）。我们还比较了PRP衍生的PPP和全血衍生的PPP（2 100×5分钟）之间由RIS诱导的MAs。

结果

与原始PRP相比，在PPP调整的PRP中，由ADP、ARA和EPI诱导的MAs降低（在2至3倍稀释率时显著，<0.05），但在PS调整的PRP中未降低（>0.05）。在PS调整的PRP中，由RIS诱导的MA降低（在所有稀释率时均显著，<0.05），但在PPP调整的PRP中未降低（>0.05）。在PS调整的PRP和PPP调整的PRP中，由COL诱导的MA没有变化（>0.05）。与PRP衍生的PPP相比，全血衍生的PPP（2 100×5分钟）由瑞斯托霉素诱导的MA相同（>0.05）。