Qin Qian-Qian, Zhang Ling, Wang Guo-Qing
Department of Public Health Laboratory Sciences,West China School of Public Health,Sichuan University,Chengdu 610041,China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 Jan;49(1):93-97.
To develop a real-time fluorescence PCR assay for quantitative detection of in fecal samples,providing technical support for quantitative detection and deep research of
The quantitative detection method for were established by using SYBR Green Ⅰ; The standard curve was made using standards; The sensitivity,specificity,anti-interference and repeatability of this assay were analyzed; 30 human fecal samples were detected by the established method.
The standard curve showed a good linear relationship with =0.999; The sensitivity of this assay reached to 1.0×10CFU/mL; This method could amplify specifically,not affected by other intestinal bacteria; Repeatability of intra-group and inter-group were good with the coefficient of variation of value lower than 2%; The positive rate of in 30 human fecal samples was 73%,while its value ranged from 17.40 to 31.77.
These results indicated that the established real-time PCR method for quantitative detection was simple,rapid and economical,good in sensitivity,specificity,anti-interference,repeatability and suitable for the detection of in faeces.
建立一种用于粪便样本中[具体检测对象]定量检测的实时荧光PCR方法，为[具体检测对象]的定量检测及深入研究提供技术支持。
采用SYBR Green Ⅰ建立[具体检测对象]的定量检测方法；使用[具体检测对象]标准品绘制标准曲线；分析该检测方法的灵敏度、特异性、抗干扰性及重复性；用建立的方法检测30份人类粪便样本。
标准曲线呈良好线性关系，R² = 0.999；该检测方法的灵敏度达到1.0×10CFU/mL；该方法能特异性扩增[具体检测对象]，不受其他肠道细菌影响；组内和组间重复性良好，R值变异系数低于2%；30份人类粪便样本中[具体检测对象]的阳性率为73%，其R值范围为17.40至31.77。
结果表明，所建立的用于[具体检测对象]定量检测的实时PCR方法简便、快速、经济，灵敏度、特异性、抗干扰性、重复性良好，适用于粪便中[具体检测对象]的检测。
