Gai Kai, Ge Yu, Liu Dapeng, Zhang He, Cong Bailin, Guo Shihao, Liu Yizheng, Xing Kai, Qi Xiaolong, Wang Xiangguo, Xiao Longfei, Long Cheng, Guo Yong, Chen Li, Sheng Xihui
Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Poult Sci. 2023 Nov;102(11):103035. doi: 10.1016/j.psj.2023.103035. Epub 2023 Aug 18.
Intramuscular fat (IMF) is an important factor affecting chicken quality. However, the age-related mechanism of IMF deposition has not yet been elucidated. In this study, the IMF, phospholipids (PL), triglycerides (TG), and fatty acid (FA) content in the breast muscle of Beijing-You chicken (BJY) at 1, 56, 98, and 120 d of age was measured, and mRNA and miRNA sequencing was integrated to explore the regulatory genes of IMF deposition. The results showed that the IMF content of BJY at 1 d of age was significantly higher than that at later stage of birth (P < 0.05). The transcriptome sequencing results showed that 7, 225 differentially expressed genes (DEGs) and 243 differentially expressed miRNAs (DE-miRNAs) were identified. The cluster analysis showed that the expression of DEGs and DE-miRNAs at 1 d of age was significantly different from that at later stages of birth. Furthermore, a potential mRNA-miRNA regulatory network related to IMF deposition was established by weighted gene co-expression network analysis (WGCNA); gga-miR-29c-3p-PIK3R1, gga-miR-6701-3p-PTEN, gga-miR-363-3p-PTEN, gga-miR-1563-WWP1, gga-miR-449c/d-5p-TRAF6, and gga-miR-6701-3p-BMPR1B were identified as key mRNA-miRNA pairs for the regulation of IMF deposition. These results will help elucidate the mechanism of IMF formation mediated by miRNAs in chickens, and provide a theoretical foundation for the genetic improvement of broiler meat quality.
肌内脂肪（IMF）是影响鸡肉品质的重要因素。然而，IMF沉积的年龄相关机制尚未阐明。本研究测定了北京油鸡（BJY）1日龄、56日龄、98日龄和120日龄时胸肌中IMF、磷脂（PL）、甘油三酯（TG）和脂肪酸（FA）的含量，并结合mRNA和miRNA测序来探索IMF沉积的调控基因。结果表明，BJY 1日龄时的IMF含量显著高于出生后期（P < 0.05）。转录组测序结果显示，共鉴定出7225个差异表达基因（DEGs）和243个差异表达miRNA（DE-miRNAs）。聚类分析表明，1日龄时DEGs和DE-miRNAs的表达与出生后期显著不同。此外，通过加权基因共表达网络分析（WGCNA）建立了一个与IMF沉积相关的潜在mRNA-miRNA调控网络；gga-miR-29c-3p-PIK3R1、gga-miR-6701-3p-PTEN、gga-miR-363-3p-PTEN、gga-miR-1563-WWP1、gga-miR-449c/d-5p-TRAF6和gga-miR-6701-3p-BMPR1B被鉴定为调控IMF沉积的关键mRNA-miRNA对。这些结果将有助于阐明鸡中miRNAs介导的IMF形成机制，并为肉鸡品质的遗传改良提供理论基础。
