Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921, Singapore.
Acta Derm Venereol. 2018 Aug 29;98(8):762-765. doi: 10.2340/00015555-2963.
Carbonic anhydrases are ubiquitously expressed enzymes that reversibly hydrate carbon dioxide to bicarbonate and protons. While the main function of carbonic anhydrases is to regulate pH and osmotic balance, their involvement in other physiological processes remains to be explored. This study analysed changes in mRNA and protein levels of carbonic anhydrase II in human primary keratinocytes treated with various toll-like receptor agonists and cytokines. A significant upregulation of carbonic anhydrase II at the mRNA and protein levels was observed upon treatment with polyinosinic-polycytidylic acid, a toll-like receptor 3 agonist. Furthermore, in agreement with the increased expression of carbonic anhydrase II in atopic dermatitis skin, carbonic anhydrase II was upregulated by the Th2 cytokines interleukins -4 and -13. In conclusion, these results suggest a potential role of carbonic anhydrase II in Th2-dependent and toll-like receptor 3-induced pathways in inflammatory skin conditions.
碳酸酐酶是广泛表达的酶，可将二氧化碳可逆地水合为碳酸氢根和质子。虽然碳酸酐酶的主要功能是调节 pH 值和渗透平衡，但它们在其他生理过程中的参与仍有待探索。本研究分析了用各种 Toll 样受体激动剂和细胞因子处理的人原代角质形成细胞中碳酸酐酶 II 的 mRNA 和蛋白水平的变化。用 Toll 样受体 3 激动剂聚肌苷酸-聚胞苷酸处理后，碳酸酐酶 II 的 mRNA 和蛋白水平显著上调。此外，与特应性皮炎皮肤中碳酸酐酶 II 表达增加一致，Th2 细胞因子白细胞介素-4 和白细胞介素-13 上调了碳酸酐酶 II 的表达。总之，这些结果表明碳酸酐酶 II 在 Th2 依赖性和 Toll 样受体 3 诱导的炎症性皮肤疾病途径中可能具有作用。
