• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

采用 DIA 质谱技术对年轻和老年皮肤进行定量蛋白质组学分析，揭示了新的与皮肤衰老相关的蛋白质。

Quantitative proteomics analysis of young and elderly skin with DIA mass spectrometry reveals new skin aging-related proteins.

机构信息

Department of Dermatology of First Affiliated Hospital, and Institute of Dermatology, Anhui Medical University, Hefei, Anhui, China.

Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, Hefei, Anhui, China.

出版信息

Aging (Albany NY). 2020 Jun 29;12(13):13529-13554. doi: 10.18632/aging.103461.

DOI:10.18632/aging.103461
PMID:32602849
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7377841/
Abstract

Skin aging is a specific manifestation of the physiological aging process that occurs in virtually all organisms. In this study, we used data independent acquisition mass spectrometry to perform a comparative analysis of protein expression in volar forearm skin samples from of 20 healthy young and elderly Chinese individuals. Our quantitative proteomic analysis identified a total of 95 differentially expressed proteins (DEPs) in aged skin compared to young skin. Enrichment analyses of these DEPs (57 upregulated and 38 downregulated proteins) based on the GO, KEGG, and KOG databases revealed functional clusters associated with immunity and inflammation, oxidative stress, biosynthesis and metabolism, proteases, cell proliferation, cell differentiation, and apoptosis. We also found that GAPDH, which was downregulated in aged skin samples, was the top hub gene in a protein-protein interaction network analysis. Some of the DEPs identified herein had been previously correlated with aging of the skin and other organs, while others may represent novel age-related entities. Our non-invasive proteomics analysis of human epidermal proteins may guide future research on skin aging to help develop treatments for age-related skin conditions and rejuvenation.

摘要

皮肤衰老（Skin aging）是几乎所有生物体生理衰老过程中的一种特定表现。在这项研究中，我们使用非依赖性采集质谱技术（data independent acquisition mass spectrometry）对 20 名健康的中国年轻和老年个体的前臂掌侧皮肤样本中的蛋白质表达进行了比较分析。我们的定量蛋白质组学分析鉴定出与年轻皮肤相比，老年皮肤中有总共 95 个差异表达蛋白（differentially expressed proteins，DEPs）。根据 GO、KEGG 和 KOG 数据库对这些 DEPs（57 个上调蛋白和 38 个下调蛋白）进行的富集分析，揭示了与免疫和炎症、氧化应激、生物合成和代谢、蛋白酶、细胞增殖、细胞分化和细胞凋亡相关的功能簇。我们还发现，在老年皮肤样本中下调的 GAPDH 是蛋白质-蛋白质相互作用网络分析中的顶级枢纽基因。本文鉴定的一些 DEPs 先前与皮肤和其他器官的衰老有关，而另一些则可能代表新的与年龄相关的实体。我们对人类表皮蛋白的非侵入性蛋白质组学分析可能指导未来的皮肤衰老研究，以帮助开发针对与年龄相关的皮肤状况和年轻化的治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/1816f6b6879e/aging-12-103461-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/080c06207039/aging-12-103461-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/15111e342fdb/aging-12-103461-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/be39ab2c0956/aging-12-103461-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/bbd95d033235/aging-12-103461-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/a5d3750e517f/aging-12-103461-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/f3ed6f2d62e5/aging-12-103461-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/e6585cf71fde/aging-12-103461-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/dbe2f7ff5e71/aging-12-103461-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/193b67d17ac7/aging-12-103461-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/1816f6b6879e/aging-12-103461-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/080c06207039/aging-12-103461-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/15111e342fdb/aging-12-103461-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/be39ab2c0956/aging-12-103461-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/bbd95d033235/aging-12-103461-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/a5d3750e517f/aging-12-103461-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/f3ed6f2d62e5/aging-12-103461-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/e6585cf71fde/aging-12-103461-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/dbe2f7ff5e71/aging-12-103461-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/193b67d17ac7/aging-12-103461-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4136/7377841/1816f6b6879e/aging-12-103461-g010.jpg

相似文献

1
Quantitative proteomics analysis of young and elderly skin with DIA mass spectrometry reveals new skin aging-related proteins.采用 DIA 质谱技术对年轻和老年皮肤进行定量蛋白质组学分析，揭示了新的与皮肤衰老相关的蛋白质。
Aging (Albany NY). 2020 Jun 29;12(13):13529-13554. doi: 10.18632/aging.103461.
2
Mass spectrometry-based proteomics reveals the distinct nature of the skin proteomes of photoaged compared to intrinsically aged skin.基于质谱的蛋白质组学揭示了光老化皮肤与内源性老化皮肤的皮肤蛋白质组的明显不同特性。
Int J Cosmet Sci. 2019 Apr;41(2):118-131. doi: 10.1111/ics.12513. Epub 2019 Apr 8.
3
Quantitative proteomic analysis of gingival crevicular fluids to identify novel biomarkers of gingival recession in orthodontic patients.定量蛋白质组学分析牙龈沟液，以鉴定正畸患者牙龈退缩的新型生物标志物。
J Proteomics. 2022 Aug 30;266:104647. doi: 10.1016/j.jprot.2022.104647. Epub 2022 Jun 30.
4
Noninvasive analysis of skin proteins in healthy Chinese subjects using an Orbitrap Fusion Tribrid mass spectrometer.采用 Orbitrap Fusion Tribrid 质谱仪对健康中国受试者的皮肤蛋白质进行非侵入性分析。
Skin Res Technol. 2019 Jul;25(4):424-433. doi: 10.1111/srt.12668. Epub 2019 Jan 18.
5
Identification of the proteomic variations of invasive relative to non-invasive non-functional pituitary adenomas.侵袭性相对于非侵袭性无功能垂体腺瘤的蛋白质组学差异鉴定。
Electrophoresis. 2014 Aug;35(15):2184-94. doi: 10.1002/elps.201300590.
6
Analysis of gene expression dynamics revealed delayed and abnormal epidermal repair process in aged compared to young skin.基因表达动力学分析显示，与年轻皮肤相比，老年皮肤的表皮修复过程延迟且异常。
Arch Dermatol Res. 2015 May;307(4):351-64. doi: 10.1007/s00403-015-1551-5. Epub 2015 Mar 6.
7
Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics.由内在或外在过程引起的皮肤老化，通过功能蛋白质组学进行表征。
Proteomics. 2016 Oct;16(20):2718-2731. doi: 10.1002/pmic.201600141. Epub 2016 Sep 12.
8
Characterization of Odontogenic Differentiation from Human Dental Pulp Stem Cells Using TMT-Based Proteomic Analysis.基于 TMT 技术的蛋白质组学分析鉴定牙髓干细胞的牙源性分化特征。
Biomed Res Int. 2020 Dec 10;2020:3871496. doi: 10.1155/2020/3871496. eCollection 2020.
9
Proteomic Analysis Reveals Differential Expression Profiles in Idiopathic Pulmonary Fibrosis Cell Lines.蛋白质组学分析揭示特发性肺纤维化细胞系中的差异表达谱。
Int J Mol Sci. 2022 May 1;23(9):5032. doi: 10.3390/ijms23095032.
10
Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation.基于iTRAQ技术的刚地弓形虫卵囊孢子化过程的全球蛋白质组学分析。
J Proteomics. 2016 Oct 4;148:12-9. doi: 10.1016/j.jprot.2016.07.010. Epub 2016 Jul 12.

引用本文的文献

1
Study on the Polarization of Astrocytes in the Optic Nerve Head of Rats Under High Intraocular Pressure: In Vitro.高眼压下大鼠视神经乳头星形胶质细胞极化的体外研究
Bioengineering (Basel). 2025 Jan 23;12(2):104. doi: 10.3390/bioengineering12020104.
2
The role of multiomics in revealing the mechanism of skin repair and regeneration.多组学在揭示皮肤修复和再生机制中的作用。
Front Pharmacol. 2025 Jan 17;16:1497988. doi: 10.3389/fphar.2025.1497988. eCollection 2025.
3
Evaluation of Collagen Dermal Filler with Lidocaine for the Correction of Nasolabial Folds: A Randomized, Double-Blind, Multicenter Clinical Trial.

本文引用的文献

1
The role of lipid metabolism in aging, lifespan regulation, and age-related disease.脂质代谢在衰老、寿命调节及年龄相关疾病中的作用。
Aging Cell. 2019 Dec;18(6):e13048. doi: 10.1111/acel.13048. Epub 2019 Sep 27.
2
Rap1A promotes esophageal squamous cell carcinoma metastasis through the AKT signaling pathway.Rap1A 通过 AKT 信号通路促进食管鳞癌细胞转移。
Oncol Rep. 2019 Nov;42(5):1815-1824. doi: 10.3892/or.2019.7309. Epub 2019 Sep 12.
3
The expression of the renin-angiotensin-aldosterone system in the skin and its effects on skin physiology and pathophysiology.
含利多卡因的胶原蛋白真皮填充剂用于矫正鼻唇沟的评估：一项随机、双盲、多中心临床试验。
Clin Cosmet Investig Dermatol. 2024 Jul 8;17:1621-1631. doi: 10.2147/CCID.S447760. eCollection 2024.
4
Integration of scRNA-seq data by disentangled representation learning with condition domain adaptation.基于条件域自适应的解缠表示学习整合 scRNA-seq 数据。
BMC Bioinformatics. 2024 Mar 16;25(1):116. doi: 10.1186/s12859-024-05706-9.
5
Cellular senescence and wound healing in aged and diabetic skin.衰老和糖尿病皮肤中的细胞衰老与伤口愈合
Front Physiol. 2024 Feb 19;15:1344116. doi: 10.3389/fphys.2024.1344116. eCollection 2024.
6
Trioxidized cysteine in the aging proteome mimics the structural dynamics and interactome of phosphorylated serine.衰老蛋白质组中的三氧化半胱氨酸模拟磷酸化丝氨酸的结构动力学和相互作用组。
Aging Cell. 2024 Mar;23(3):e14062. doi: 10.1111/acel.14062. Epub 2023 Dec 18.
7
A pilot study of S100A4, S100A8/A9, and S100A12 in dilated cardiomyopathy: novel biomarkers for diagnosis or prognosis?S100A4、S100A8/A9 和 S100A12 在扩张型心肌病中的初步研究：用于诊断或预后的新型生物标志物？
ESC Heart Fail. 2024 Feb;11(1):503-512. doi: 10.1002/ehf2.14605. Epub 2023 Dec 11.
8
Combining proteomic markers to construct a logistic regression model for polycystic ovary syndrome.联合蛋白质组学标志物构建多囊卵巢综合征的逻辑回归模型。
Front Endocrinol (Lausanne). 2023 Oct 3;14:1227252. doi: 10.3389/fendo.2023.1227252. eCollection 2023.
9
Inhibition of Polyamine Catabolism Reduces Cellular Senescence.抑制多胺分解代谢可减少细胞衰老。
Int J Mol Sci. 2023 Aug 29;24(17):13397. doi: 10.3390/ijms241713397.
10
One-year longitudinal study of the stratum corneum proteome of retinol and all-trans-retinoic acid treated human skin: an orchestrated molecular event.视黄醇和全反式维 A 酸处理人皮肤角质层蛋白质组的一年纵向研究：一个协调的分子事件。
Sci Rep. 2023 Jul 11;13(1):11196. doi: 10.1038/s41598-023-37750-5.
肾素-血管紧张素-醛固酮系统在皮肤中的表达及其对皮肤生理学和病理生理学的影响。
J Physiol Pharmacol. 2019 Jun;70(3). doi: 10.26402/jpp.2019.3.01. Epub 2019 Sep 18.
4
Zebrafish modeling reveals that SPINT1 regulates the aggressiveness of skin cutaneous melanoma and its crosstalk with tumor immune microenvironment.斑马鱼模型研究揭示 SPINT1 调控皮肤黑色素瘤的侵袭性及其与肿瘤免疫微环境的相互作用。
J Exp Clin Cancer Res. 2019 Sep 13;38(1):405. doi: 10.1186/s13046-019-1389-3.
5
KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2α.KCTD1：通过与转录因子 AP2α 相互作用调节脂肪生成的新型调节剂。
Biochim Biophys Acta Mol Cell Biol Lipids. 2019 Dec;1864(12):158514. doi: 10.1016/j.bbalip.2019.08.010. Epub 2019 Aug 26.
6
Decreased expression of GPC1 in human skin keratinocytes and epidermis during ageing.人皮肤角质形成细胞和表皮中 GPC1 的表达随年龄增长而降低。
Exp Gerontol. 2019 Oct 15;126:110693. doi: 10.1016/j.exger.2019.110693. Epub 2019 Aug 17.
7
New Insight in Noninvasive Rejuvenation: The Role of a Rhodamine-Intense Pulsed Light System.非侵入性嫩肤新见解：罗丹明强脉冲光系统的作用
Photobiomodul Photomed Laser Surg. 2019 Sep;37(9):539-543. doi: 10.1089/photob.2019.4626. Epub 2019 Aug 5.
8
The cysteine-rich domain of synaptosomal-associated protein of 23 kDa (SNAP-23) regulates its membrane association and regulated exocytosis from mast cells.突触相关蛋白 23kDa 的富含半胱氨酸结构域（SNAP-23）调节其与膜的结合以及肥大细胞中受调控的胞吐作用。
Biochim Biophys Acta Mol Cell Res. 2019 Oct;1866(10):1618-1633. doi: 10.1016/j.bbamcr.2019.06.015. Epub 2019 Jun 29.
9
Destabilization of the human RED-SMU1 splicing complex as a basis for host-directed antiinfluenza strategy.作为宿主定向抗流感策略基础的人 RED-SMU1 剪接复合物的失稳。
Proc Natl Acad Sci U S A. 2019 May 28;116(22):10968-10977. doi: 10.1073/pnas.1901214116. Epub 2019 May 10.
10
The Immune Functions of α Acid Glycoprotein.α 酸糖蛋白的免疫功能。
Curr Protein Pept Sci. 2019;20(6):505-524. doi: 10.2174/1389203720666190405101138.