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[多杀性巴氏杆菌的分子分型方法——综述]

[Molecular typing methods for Pasteurella multocida-A review].

作者信息

Peng Zhong, Liang Wan, Wu Bin

出版信息

Wei Sheng Wu Xue Bao. 2016 Oct 4;56(10):1521-9.

Abstract

Pasteurella multocida is an important gram-negative pathogenic bacterium that could infect wide ranges of animals. Humans could also be infected by P. multocida via animal bite or scratching. Current typing methods for P. multocida include serological typing methods and molecular typing methods. Of them, serological typing methods are based on immunological assays, which are too complicated for clinical bacteriological studies. However, the molecular methods including multiple PCRs and multilocus sequence typing (MLST) methods are more suitable for bacteriological studies of P. multocida in clinic, with their simple operation, high efficiency and accurate detection compared to the traditional serological typing methods, they are therefore widely used. In the current review, we briefly describe the molecular typing methods for P. multocida. Our aim is to provide a knowledge-foundation for clinical bacteriological investigation especially the molecular investigation for P. multocida.

摘要

多杀性巴氏杆菌是一种重要的革兰氏阴性病原菌,可感染多种动物。人类也可能通过动物咬伤或抓伤而感染多杀性巴氏杆菌。目前多杀性巴氏杆菌的分型方法包括血清学分型方法和分子分型方法。其中,血清学分型方法基于免疫测定,对于临床细菌学研究来说过于复杂。然而,包括多重PCR和多位点序列分型(MLST)方法在内的分子方法更适合临床上多杀性巴氏杆菌的细菌学研究,与传统血清学分型方法相比,它们操作简单、效率高且检测准确,因此被广泛应用。在本综述中,我们简要描述了多杀性巴氏杆菌的分子分型方法。我们的目的是为临床细菌学调查,特别是多杀性巴氏杆菌的分子调查提供知识基础。

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