高蔗糖含量溶液中的 DPPC 双层膜。
DPPC Bilayers in Solutions of High Sucrose Content.
机构信息
Université de Strasbourg, CNRS, Institut Charles Sadron, UPR022, Strasbourg Cedex, France.
Université de Strasbourg, CNRS, Institut Charles Sadron, UPR022, Strasbourg Cedex, France.
出版信息
Biophys J. 2018 May 8;114(9):2165-2173. doi: 10.1016/j.bpj.2018.04.003.
Abstract
The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease in the transition enthalpy but only a minor shift in the transition temperature. Our observations can be quantitatively accounted for by a thermodynamic model that assumes partial delayed melting induced by sucrose adsorption at the membrane interface.
摘要
近年来，由于糖在生物保存领域的重要性，蔗糖溶液中脂质双层的性质受到了广泛关注。然而，关于糖-脂质相互作用的机制尚未达成共识。在这里，我们结合量热法、荧光光谱法和光学显微镜研究了蔗糖对 1,2-二月桂酰基-sn-甘油-3-磷酸胆碱双层的影响。有趣的是，我们的结果表明，相变焓显著降低，而相变温度仅略有移动。我们的观察结果可以通过一个热力学模型来定量解释，该模型假设蔗糖在膜界面的吸附导致部分延迟熔化。
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