Sugiki Mikio, Miyakawa Hiroyuki, Tahara Shoichi, Yamamoto Sumiyo, Sakamaki Narue, Sadamasu Yuki, Uematsu Yoko, Monma Kimio
Tokyo Metropolitan Institute of Public Health.
Shokuhin Eiseigaku Zasshi. 2018;59(1):30-35. doi: 10.3358/shokueishi.59.30.
A quantitative analysis by HPLC of α-glucosyltransferase-treated stevia in foods was considered. This analysis is the way which hydrolyzed α-glucosyltransferase-treated stevia in the stevioside (SS) and the rebaudioside A (RS) using a glucoamylase. Recovery (%) of α-glucosyltransferase-treated stevia, spiked at 200 mg/kg in various foods, were more than 80% and the relative standard deviations were less than 5.0% as SS and RS for the rate of collection. A qualitative analysis by LC-MS/MS was performed 36 products of commercial foods containing stevia. We quantified of 11 products in which α-glucosyltransferase-treated stevia was detected. Quantitative value was at most 180 mg/kg as SS, at most 70 mg/kg as RS.
考虑通过高效液相色谱法（HPLC）对食品中α-葡萄糖基转移酶处理的甜菊糖苷进行定量分析。该分析方法是使用糖化酶水解甜菊糖苷（SS）和莱鲍迪苷A（RS）中经α-葡萄糖基转移酶处理的甜菊糖苷。在各种食品中添加量为200 mg/kg的经α-葡萄糖基转移酶处理的甜菊糖苷的回收率（%）超过80%，作为SS和RS的收集率的相对标准偏差小于5.0%。对36种含有甜菊糖苷的市售食品进行了液相色谱-串联质谱法（LC-MS/MS）定性分析。我们对11种检测到经α-葡萄糖基转移酶处理的甜菊糖苷的产品进行了定量。作为SS的定量值最高为180 mg/kg，作为RS的定量值最高为70 mg/kg。
