Miyakawa Hiroyuki, Sugiki Mikio, Tahara Shoichi, Yamamoto Sumiyo, Yamajima Yukiko, Uematsu Yoko, Monma Kimio
Tokyo Metropolitan Institute of Public Health.
Shokuhin Eiseigaku Zasshi. 2018;59(5):213-219. doi: 10.3358/shokueishi.59.213.
A LC-MS/MS-based screening method was developed for stevia sweetener in processed foods. After extraction of stevia sweetener from processed foods by dialysis, the dialysate was diluted with water, and stevia sweetener was measured by LC-MS/MS. Recovery from 5 kinds of processed foods spiked with 10 mg/kg of stevioside （SS）, 10 mg/kg of rebaudioside A （RS）, or 100 mg/kg of α-glu-cosyltransferase-treated stevia （Gts） product was excellent, and no interfering peak was observed. Thirty-six commercial processed foods indicated as containing "stevia" were analyzed using this established method. Among them, 33 contained SS, 33 contained RS, and 11 contained Gts. Five products contained both stevia extract and Gts.
建立了一种基于液相色谱-串联质谱（LC-MS/MS）的加工食品中甜菊糖苷甜味剂的筛查方法。通过透析从加工食品中提取甜菊糖苷甜味剂后，用去离子水稀释透析液，然后用LC-MS/MS测定甜菊糖苷甜味剂。在添加了10mg/kg甜菊苷（SS）、10mg/kg莱鲍迪苷A（RS）或100mg/kgα-葡萄糖基转移酶处理的甜菊糖苷（Gts）产品的5种加工食品中的回收率良好，未观察到干扰峰。使用该既定方法对36种标明含有“甜菊”的商业加工食品进行了分析。其中，33种含有SS，33种含有RS，11种含有Gts。5种产品同时含有甜菊提取物和Gts。
