Department of Molecular Biology, Umeå University, 90187, Umea, Sweden.
The Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, 90187, Umea, Sweden.
Sci Rep. 2018 May 9;8(1):7434. doi: 10.1038/s41598-018-25308-9.
Interactions between bacteria and colon cancer cells influence the transcription of the host cell. Yet is it undetermined whether the bacteria itself or the communication between the host and bacteria is responsible for the genomic changes in the eukaryotic cell. Now, we have investigated the genomic and epigenetic consequences of co-culturing colorectal carcinoma cells with membrane vesicles from pathogenic bacteria Vibrio cholerae and non-pathogenic commensal bacteria Escherichia coli. Our study reveals that membrane vesicles from pathogenic and commensal bacteria have a global impact on the gene expression of colon-carcinoma cells. The changes in gene expression correlate positively with both epigenetic changes and chromatin accessibility of promoters at transcription start sites of genes induced by both types of membrane vesicles. Moreover, we have demonstrated that membrane vesicles obtained only from V. cholerae induced the expression of genes associated with epithelial cell differentiation. Altogether, our study suggests that the observed genomic changes in host cells might be due to specific components of membrane vesicles and do not require communication by direct contact with the bacteria.
细菌与结肠癌细胞之间的相互作用会影响宿主细胞的转录。然而，尚不确定是细菌本身还是宿主与细菌之间的通讯导致真核细胞的基因组发生变化。现在，我们已经研究了将结肠直肠癌细胞与致病性霍乱弧菌和非致病性共生菌大肠杆菌的膜泡共同培养对基因组和表观遗传的影响。我们的研究表明，致病性和共生菌的膜泡对结肠癌细胞的基因表达具有全局影响。基因表达的变化与两种膜泡诱导的启动子的表观遗传变化和染色质可及性呈正相关。此外，我们已经证明，仅从霍乱弧菌获得的膜泡可诱导与上皮细胞分化相关的基因表达。总的来说，我们的研究表明，宿主细胞中观察到的基因组变化可能是由于膜泡的特定成分引起的，而不需要与细菌直接接触进行通讯。
