Witwer Kenneth W
Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
F1000Res. 2018 Feb 28;7:244. doi: 10.12688/f1000research.14060.1. eCollection 2018.
A 2017 study reported that "Plant miRNAs found in human circulating system provide evidences of cross kingdom RNAi". Analysis of two human blood plasma sequencing datasets was said to provide evidence for uptake of plant miRNAs into human plasma. The results were also purportedly inconsistent with contamination. Sequences from public datasets and miRNA databases were compared with results downloaded from the website of the reporting journal. Only one putative plant miRNA ("peu-MIR2910) mapped consistently above background, and this sequence is found with 100% identity in a human rRNA. Several other rarer but consistently mapped putative plant miRNAs also have 100% or near 100% matches to human transcripts or genomic sequences, and some do not appear to map to plant genomes at all. Reanalysis of public data suggests that dietary plant xenomiR uptake is not supported, but instead confirms previous findings that detection of rare plant miRNAs in mammalian sequencing datasets is artifactual. Some putative plant miRNAs, including MIR2910 and MIR2911, may represent human sequence contamination or other artifacts in plant studies, emphasizing the need for rigorous controls and data filtering strategies when assessing possible xenomiRNAs.
2017年的一项研究报告称：“在人类循环系统中发现的植物微小RNA为跨界RNA干扰提供了证据”。据说对两个人类血浆测序数据集的分析为植物微小RNA被吸收进入人类血浆提供了证据。据称，这些结果也与污染情况不符。将公共数据集和微小RNA数据库中的序列与从该报告期刊网站下载的结果进行了比较。只有一种假定的植物微小RNA（“peu-MIR2910”）在背景之上持续映射，并且该序列在人类核糖体RNA中具有100%的同一性。其他几种较罕见但持续映射的假定植物微小RNA也与人类转录本或基因组序列具有100%或接近100%的匹配，并且有些似乎根本不映射到植物基因组。对公共数据的重新分析表明，饮食中植物外源性微小RNA的吸收得不到支持，反而证实了先前的发现，即在哺乳动物测序数据集中检测到的罕见植物微小RNA是人为造成的。一些假定的植物微小RNA，包括MIR2910和MIR2911，可能代表植物研究中的人类序列污染或其他人为因素，这强调了在评估可能的外源性微小RNA时需要严格的对照和数据过滤策略。
