Jing Ruiyong, Cao Kun, Liu Junjie, Liu Judong, Jin Jian, Liu Xiaobing, Wang Guanghua
Wei Sheng Wu Xue Bao. 2017 Jan 4;57(1):131-9.
To provide scientific data for studying the ecology of cyanophage, we studied the genetic diversity of psbA of cyanophage from paddy floodwater in northeast China and its phylogenetic positions.
Membrane separation and concentration of cyanophage, PCR-cloning-sequencing were applied to study the diversity of psbA of cyanophage from paddy floodwater in northeast China.
In total 17 psbA sequences of cyanophage were obtained. Novel cyanophages were found by phylogenetic analysis. Compared to those of Japanese paddy floodwater, marine and lakes, psbA gene assemblage of paddy floodwater in northeast China was significantly different.
This is the first report to study genetic diversity of cyanophage from paddy floodwater in northeast China with a molecular marker of psbA by PCR-cloning-sequencing. The novel psbA assembly of cyanophage was found in paddy floodwater in northeast China.
为研究噬藻体生态学提供科学数据，我们对中国东北地区稻田洪水水体中噬藻体的psbA基因遗传多样性及其系统发育地位进行了研究。
采用膜分离浓缩噬藻体，运用PCR-克隆-测序技术研究中国东北地区稻田洪水水体中噬藻体的psbA基因多样性。
共获得17条噬藻体psbA基因序列。通过系统发育分析发现了新型噬藻体。与日本稻田洪水水体、海洋和湖泊中的噬藻体相比，中国东北地区稻田洪水水体的psbA基因组合存在显著差异。
这是首次利用PCR-克隆-测序技术，以psbA为分子标记对中国东北地区稻田洪水水体中噬藻体的遗传多样性进行研究。在中国东北地区稻田洪水水体中发现了新型噬藻体psbA基因组合。
