Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China.
FEMS Microbiol Ecol. 2011 May;76(2):352-9. doi: 10.1111/j.1574-6941.2011.01052.x. Epub 2011 Feb 14.
Bacteriophages are ubiquitous in various environments. Our previous study revealed the diversity of the cyanophage community in paddy floodwater. In this study, the phylogeny and genetic diversity of cyanophage communities in paddy field soils were reported. The viral capsid assembly protein gene (g20) of cyanophage was amplified with the primers CPS1 and CPS8 from soil DNA extracted during two different sampling times at three sampling sites in Japan. The sequencing results indicated that about 93% of the clones were g20 genes. In total, 70 clones of g20 genes were obtained in this study, of which 69 clones were of cyanophage origin. As evaluated by g20 sequence assemblages in paddy field soils, the unifrac analyses results indicated that cyanophage communities changed among the sampling sites and times and differed from those communities detected in paddy floodwater. The phylogenetic analysis showed that the g20 sequences in paddy field soils were very diverse and distributed into Clusters α, β and ɛ, as well as four newly formed clusters. Within Clusters β and ɛ, four unique subclusters were formed from the g20 clones that were only observed in this study. These findings suggested that the cyanophage communities in paddy field soils are different from those found in freshwater, marine water and paddy floodwater.
噬菌体在各种环境中广泛存在。我们之前的研究揭示了稻田洪水水体内噬藻体群落的多样性。在本研究中，报道了稻田土壤中噬藻体群落的系统发育和遗传多样性。从日本三个采样点在两个不同的采样时间从土壤 DNA 中扩增噬藻体病毒衣壳组装蛋白基因（g20），使用引物 CPS1 和 CPS8。测序结果表明，约 93%的克隆为 g20 基因。本研究共获得 70 个 g20 基因克隆，其中 69 个为噬藻体来源。通过对稻田土壤中 g20 序列组合的评价，无差异分析结果表明，噬藻体群落随采样地点和时间而变化，与稻田洪水中检测到的群落不同。系统发育分析表明，稻田土壤中的 g20 序列非常多样化，分布在聚类 α、β和ɛ，以及四个新形成的聚类中。在聚类β和ɛ中，从本研究中仅观察到的 g20 克隆形成了四个独特的亚聚类。这些发现表明，稻田土壤中的噬藻体群落与淡水、海水和稻田洪水中的噬藻体群落不同。
