Jamaldheen Sumitha Banu, Sharma Kedar, Rani Aruna, Moholkar Vijayanand S, Goyal Arun
a Centre for Energy, Indian Institute of Technology Guwahati , Guwahati , India.
b DBT PAN-IIT Center for Bioenergy , Indian Institute of Technology Guwahati , Guwahati , India.
Prep Biochem Biotechnol. 2018;48(6):457-464. doi: 10.1080/10826068.2018.1466148. Epub 2018 Jul 9.
This study compares different types of pretreatment methods, such as thermal pretreatment at 120 °C, autoclaving, microwaving and ultrasonication in the presence of water, dilute acid (1% HSO) or dilute alkali (1% NaOH) on Sorghum stalk with respect to the holocellulose and Acid Detergent/Insoluble Lignin content. Among all the methods, pretreatment with 1% NaOH along with autoclaving at 121 °C and 15 psi for 30 min was the most effective method for Sorghum stalk. Fourier Transform Infra-Red spectroscopy analysis of this pretreated biomass showed the removal of lignin and Field Emission Scanning Electron Microscope analysis displayed enhanced surface roughness. The enzymatic hydrolysis of raw and best pretreated Sorghum stalk using recombinant endo-β-1,4-glucanase (CtCel8A) and β-1,4-glucosidase (CtBgl1A) both from Clostridium thermocellum gave glucose yields, 22.4 mg/g raw biomass and 34 mg/g pretreated biomass, respectively, resulting in 1.5-fold increase of glucose yield after the pretreatment.
本研究比较了不同类型的预处理方法，如在120°C下进行热预处理、高压灭菌、微波处理以及在水、稀酸（1% H₂SO₄）或稀碱（1% NaOH）存在的情况下进行超声处理，考察其对高粱秸秆全纤维素和酸性洗涤/酸不溶木质素含量的影响。在所有方法中，1% NaOH与在121°C、15 psi下高压灭菌30分钟相结合的预处理方法对高粱秸秆最为有效。对这种预处理后的生物质进行傅里叶变换红外光谱分析显示木质素被去除，场发射扫描电子显微镜分析显示表面粗糙度增加。使用来自嗜热栖热菌的重组内切-β-1,4-葡聚糖酶（CtCel8A）和β-1,4-葡萄糖苷酶（CtBgl1A）对未处理的和预处理效果最佳的高粱秸秆进行酶水解，葡萄糖产率分别为22.4 mg/g未处理生物质和34 mg/g预处理生物质，预处理后葡萄糖产率提高了1.5倍。
