Industry and Investment NSW, Wollongbar Primary Industries Institute, NSW, Australia.
Bioresour Technol. 2010 Sep;101(17):6718-27. doi: 10.1016/j.biortech.2010.03.116. Epub 2010 Apr 18.
The impacts of varying pretreatment parameters (temperature, time, and alkalinity) on enzymatic hydrolysis of sorghum straw were investigated. Following pretreatment, both solids and lignin content was found to be inversely proportional to the severity of the treatments. Higher temperatures and alkali strength were quintessential for maximising sugar recoveries from enzyme saccharifications. Total sugar release peaked when sorghum straw was pretreated in 2% NaOH at 121 degrees C for 60 min; representing a 5.6-fold higher yield compared to samples pretreated at 60 degrees C in the absence of alkali. Similarly, 4.3-fold increases in total sugars from samples treated with 2% NaOH at 60 degrees C for 90 min, confirmed the importance of alkali inclusion. Addition of beta-glucosidase and xylanase to saccharification mixtures enhanced reaction rates and final sugar yields, whilst reducing cellulase dosage 4-fold. Saccharification efficiency of pretreated solids approached 90% and 95% (w/w) with as little as 2.5 and 5.0 FPU cellulase/g, respectively.
研究了不同预处理参数（温度、时间和碱度）对高粱秸秆酶解的影响。预处理后，固体和木质素含量与处理的剧烈程度成反比。更高的温度和碱强度对于从酶解中最大限度地回收糖是至关重要的。当高粱秸秆在 2%NaOH 中于 121°C 下预处理 60 分钟时，总糖释放量达到峰值；与在 60°C 下无碱预处理的样品相比，产量提高了 5.6 倍。同样，在 60°C 下用 2%NaOH 处理 90 分钟的样品中总糖增加了 4.3 倍，证实了碱的加入的重要性。向糖化混合物中添加β-葡萄糖苷酶和木聚糖酶提高了反应速率和最终糖产量，同时将纤维素酶的用量减少了 4 倍。预处理后固体的糖化效率达到 90%（w/w）和 95%（w/w），分别只需 2.5 和 5.0 FPU 纤维素酶/g。
Zotero 插件