Yang M L, Yang L
Department of Ophthalmology, Peking University First Hospital, Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing 100034, China.
Zhonghua Yan Ke Za Zhi. 2018 May 11;54(5):357-362. doi: 10.3760/cma.j.issn.0412-4081.2018.05.009.
To observe the ultrastructure of lens epithelium cells in cataract associated with uveitis. A cross-sectional study. Seven patients(7 eyes) received phacoemulsification in Peking University First Hospital from August 2016 to March 2017 due to complicated cataract associated with uveitis[2 males and 5 females,with an average age of (49±20) years] and 3 patients of age-related cataract (3 eyes, females, aged 54, 71, 74 years) were enrolled. Anterior capsule samples were collected during surgery. Transmission electron microscopy was performed. The cell morphology, the proportion of cell nuclear and organelle changes and the proportion of apoptosis in cataract associated with uveitis were described statistically by ± or median (range). Spindle-shaped epithelial cells were observed in cataract associated with uveitis (100%), while 28.00%,16.67%,16.67% of spindle-shaped epithelial cells were observed in age-related cataract. The organelle changes included mitochondria swelling and damage to the tight cell junction in cataract associated with uveitis. The tight junction between two cells was damaged in different degrees, only 8.33% (0-16.67%)of the cell nuclei appeared normal, and increased chromatin density(47.07%±22.28%), nuclear pyknosis(38.02%±19.61%) and nuclear fragmentation (9.96%±8.10%) were observed in cataract secondary to uveitis. The apoptosis rate was (48.16%±26.66%) in cataract associated with uveitis and correlated to the duration of intraocular inflammation. While the apoptosis rate were 0, 8.33%, 0 in age-related cataract patients. No autophagosome was observed in cataract associated with uveitis. Ultrastructure changes existed in lens epithelium cells in cataract associated with uveitis. The increased rate of apoptosis and inhibition of autophagy could be the possible mechanism of cataract formation in uveitic eyes. .
观察葡萄膜炎相关性白内障晶状体上皮细胞的超微结构。一项横断面研究。2016年8月至2017年3月，7例（7只眼）因合并葡萄膜炎的复杂性白内障在北京大学第一医院接受了超声乳化手术[2例男性和5例女性，平均年龄(49±20)岁]，并纳入3例年龄相关性白内障患者（3只眼，女性，年龄分别为54、71、74岁）。手术中采集前囊膜样本。进行透射电子显微镜检查。采用均值±标准差或中位数（范围）对葡萄膜炎相关性白内障的细胞形态、细胞核及细胞器变化比例和凋亡比例进行统计学描述。在葡萄膜炎相关性白内障中观察到纺锤形上皮细胞（100%），而在年龄相关性白内障中观察到纺锤形上皮细胞的比例分别为28.00%、16.67%、16.67%。细胞器变化包括葡萄膜炎相关性白内障中的线粒体肿胀和紧密细胞连接受损。两个细胞之间的紧密连接有不同程度的损伤，葡萄膜炎继发性白内障中仅8.33%（0 - 16.67%）的细胞核外观正常，且观察到染色质密度增加（47.07%±22.28%）、核固缩（38.02%±19.61%）和核碎裂（9.96%±8.10%）。葡萄膜炎相关性白内障的凋亡率为(48.16%±26.66%)，且与眼内炎症持续时间相关。而年龄相关性白内障患者的凋亡率分别为0、8.33%、0。在葡萄膜炎相关性白内障中未观察到自噬体。葡萄膜炎相关性白内障的晶状体上皮细胞存在超微结构改变。凋亡率增加和自噬抑制可能是葡萄膜炎性眼白内障形成的可能机制。
