Li Guang Hao, Liu Ping Ping, Zhao Bin, Dong Shu Ting, Liu Peng, Zhang Ji Wang, Tian Cui Xia, He Zai Ju
College of Agronomy, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2017 Feb;28(2):571-580. doi: 10.13287/j.1001-9332.201702.007.
In an soil column experiment with Zhengdan 958 (a summer maize cultivar planted widely in China), treatments of three water levels,severe water stress W which the soil moisture kept (35±5)% of the field capacity, mild water stress W which was (55±5)%,normal water W which was (75±5)%, and four levels of controlled release urea fertilizer (N, N was 150 kg N·hm,N was 225 kg N·hm and N was 300 kg N·hm) were included to study the interactive effects of water and controlled release urea on yield and leaf senescence characteristics of summer maize. The results showed that the coupling of water and controlled release urea had significant effects on increasing yield, delaying the senescence and keeping the high efficiency of the functional leaves. Under the same nitrogen condition, yield, LAI, chlorophyll content and the activities of SOD, POD, CAT and soluble protein content in summer maize ear leaf were significantly increased with more water supplying, and the content of MDA decreased significantly. Under the condition of the same moisture, these indicators were also significantly increased with the increasing nitrogen application and MDA content was reduced significantly. However, these indicators (except MDA) of WN, WN and WN treatments were maintained at a higher level and the MDA content was lo-wer compared with other treatments despite the fact that there were no significant difference among these three treatments, which indicated that the interactive effects of water and controlled release urea had an important role in maintaining the function of ear leaf, delaying the leaf senescence, and was beneficial to the photosynthates production and obtaining higher yield of summer maize. Integrating the yield, LAI, chlorophyll content, various protective enzymes activity, MDA and soluble protein content, controlled release urea application rate of 225 kg N·hm was the best treatment as the soil moisture content was (75±5)% of field capacity. Continuous increase in the nitrogen application could not enhance the activities of protective enzymes, oppositely, it could cause the decline of protective enzymes activities and the increase of MDA content rapidly and speed up plants translation to senescence, which was not conductive to the efficient use of nitrogen. We suggested that coupling controlled release urea application rate of 300 kg N·hm with soil moisture content of (55±5)% of field capacity was optimum.
在以郑单958(中国广泛种植的夏玉米品种)为材料的土柱试验中,设置了3个水分水平处理,即土壤水分保持在田间持水量的(35±5)%的重度水分胁迫W、(55±5)%的轻度水分胁迫W、(75±5)%的正常水分W,以及4个控释尿素肥料水平(N1,施氮量为150 kg N·hm²;N2,施氮量为225 kg N·hm²;N3,施氮量为300 kg N·hm²),研究水分与控释尿素对夏玉米产量及叶片衰老特性的交互作用。结果表明,水分与控释尿素的耦合对提高产量、延缓衰老和维持功能叶的高效性有显著影响。在相同施氮条件下,夏玉米穗位叶的产量、叶面积指数(LAI)、叶绿素含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和可溶性蛋白含量随着供水量增加而显著提高,丙二醛(MDA)含量显著降低。在相同水分条件下,随着施氮量增加这些指标也显著提高,MDA含量显著降低。然而,WN1、WN2和WN3处理的这些指标(除MDA外)维持在较高水平,且MDA含量低于其他处理,尽管这3个处理之间差异不显著,这表明水分与控释尿素的交互作用对维持穗位叶功能、延缓叶片衰老具有重要作用,有利于光合产物的生产及夏玉米获得较高产量。综合产量、LAI、叶绿素含量、各种保护酶活性、MDA和可溶性蛋白含量来看,当土壤含水量为田间持水量的(75±5)%时,控释尿素施用量为225 kg N·hm²是最佳处理。持续增加施氮量并不能增强保护酶活性,相反,会导致保护酶活性迅速下降、MDA含量增加,加速植株向衰老转变,不利于氮素的高效利用。建议控释尿素施用量300 kg N·hm²与土壤含水量为田间持水量的(55±5)%相耦合为最佳。