Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, New South Wales Health Pathology, NSW, Australia; Westmead Hospital, Westmead, NSW, Australia.
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, New South Wales Health Pathology, NSW, Australia; Westmead Hospital, Westmead, NSW, Australia.
Pathology. 2018 Jun;50(4):450-454. doi: 10.1016/j.pathol.2017.11.093. Epub 2018 May 8.
Despite the reported elimination of measles virus in Australia, importation of cases from endemic countries continues to lead to secondary local transmission and outbreaks. Rapid laboratory confirmation of measles is paramount for individual patient management and outbreak responses. Further, it is important to rapidly distinguish infection from wild-type virus or vaccine strains to guide public health responses. We developed a high throughput, TaqMan-based multiplex reverse-transcription-polymerase chain reaction (PCR) assay using the BD MAX platform (Becton Dickinson) that simultaneously detects measles virus and differentiates between wild-type and vaccine strains without the need for sequencing.
尽管澳大利亚已报告消灭了麻疹病毒，但麻疹病例仍不断从流行国家输入，导致了继发的本地传播和暴发。快速实验室确认麻疹对个体患者的管理和暴发应对至关重要。此外，快速区分感染来自野生型病毒还是疫苗株，对于指导公共卫生应对措施也很重要。我们使用 BD MAX 平台（Becton Dickinson）开发了一种高通量、基于 TaqMan 的多重逆转录-聚合酶链反应（PCR）检测方法，该方法可同时检测麻疹病毒，并区分野生型和疫苗株，而无需测序。
