A lead analysis based on amine functionalized bimodal mesoporous silica nanoparticles in human biological samples by ultrasound assisted-ionic liquid trap-micro solid phase extraction.

A simple and applied method based on ultrasound assisted-Ionic liquid trap-micro solid phase extraction (USA-ILT-μSPE) was demonstrated for preconcentration/separation of lead (Pb) in human blood and urine samples. By proposed method, the amine-functionalized bimodal mesoporous silica nanoparticles (NH-UVM) as solid phase sorbent was used for lead analysis in human biological samples at pH ≈ 7.0. After shaking/centrifuging of sample, the Pb (II) was extracted with NH-UVM without any chelating agents, trapped in ionic liquid phase and settled down in conical centrifuge tube as a residual solution [Pb→:NH-UVM]. Finally, the lead ions was back extracted from residual solution and determined by atom trap flame atomic absorption spectrometry (AT-FAAS). Under the optimal conditions, the linear range (LR), limit of detection (LOD) and preconcentration factor (PF) were obtained (10-950 μg L; 2.0-210 μg L), (2.8 μg L; 0.6 μg L) and (19.8; 99.7) for human blood and urine samples, respectively (% RSD < 1, intra-day). The reusability and adsorption capacity of the sorbent were also obtained 24 and 213.8 mg g, respectively. Validation of methodology was confirmed by certified reference materials (NIST, CRM).