Department of Biotechnology, Haldia Institute of Technology, Haldia 721 657, Hatiberia, West Bengal, India; Department of Zoology, Visva-Bharati, Santiniketan 731 235, West Bengal, India.
Department of Biotechnology, Haldia Institute of Technology, Haldia 721 657, Hatiberia, West Bengal, India.
Bioresour Technol. 2018 Sep;263:280-288. doi: 10.1016/j.biortech.2018.04.122. Epub 2018 May 1.
Three bacteria (MS I, II and III) i.e., Pseudomonas aeruginosa (KY781886), Enterobactor ludwigii (KX881423) and Enterobacter cloacae (KX881513) isolated from domestic sewage were identified on the basis of 16S rDNA sequencing and are capable to growth in the presence of organo-phosphorous pesticide (chlorpyrifos). The mega plasmid size >23 kb was found in MS I and III. Biosurfactants of the significant amount were produced by three isolates. The ability of the isolates to degrade pesticide over 3 days in the presence of pesticides containing chlorpyrifos as the active component was estimated. Results of UV-visible, FTIR spectroscopy and GC-MS studies confirmed the removal of chlorpyrifos rather than degradation. Pesticide uptake results showed chlorpyrifos in intracellular components and bound to the cell surface in its native state. Removal of pesticide from soil was also recorded by these bacteria. Microbial treated pesticide did not have any effect on Vigna radita seedlings and goat erythrocytes.
从生活污水中分离出的三株细菌（MS I、II 和 III），即绿脓假单胞菌（KY781886）、产酸克雷伯氏菌（KX881423）和阴沟肠杆菌（KX881513），通过 16S rDNA 测序进行了鉴定，并且能够在有机磷农药（毒死蜱）存在的情况下生长。在 MS I 和 III 中发现了大小>23kb 的大型质粒。三种分离株均产生了大量的生物表面活性剂。在含有毒死蜱作为活性成分的农药存在的情况下，估计了分离株在 3 天内降解农药的能力。UV-可见分光光度法、傅里叶变换红外光谱法和 GC-MS 研究结果证实了氯吡硫磷的去除而不是降解。农药摄取结果表明，氯吡硫磷存在于细胞内成分中，并以其天然状态结合在细胞表面。这些细菌也从土壤中去除了农药。微生物处理过的农药对豇豆幼苗和山羊红细胞没有任何影响。
