College of Chemistry and Environmental Science, Hebei University, Baoding City, Hebei Province, 071002, PR China.
College of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
Ecotoxicol Environ Saf. 2018 Sep 15;159:190-197. doi: 10.1016/j.ecoenv.2018.04.047. Epub 2018 May 21.
Using sequential soil and liquid culture enrichments with cyprodinil as the sole carbon source, a Gram-negative cyprodinil-degrader from cyprodinil-polluted agricultural soil was isolated. The sequencing analysis of 16 S rRNA indicated that the strain showed 99% homology to Acinetobacter sp. The strain could effectively degrade cyprodinil at the neutral condition. At the initial concentrations of 10, 20, 50, 100, 150 and 200 mg L in minimal medium, cyprodinil was degraded by 10, 20, 49.3, 64.2, 57 and 24 mg L within 14 days, respectively. Two metabolites (4-cyclopropyl-6-methyl-2-pyrimidpyridine amine and monohydroxylated para-substitution) were identified using high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS/MS). A biodegradation pathway involving imines hydrolysis and monohydroxyl substitution on benzene ring was proposed on basis of the identified metabolites. Acinetobacter sp. would have a potential application in bioremediation of cyprodinil-contaminated soil, and the strain might have important implications in detoxification and bioremediation of pyrimidine analogues.
采用序贯土壤和液体培养富集，以 cyprodinil 作为唯一碳源，从受 cyprodinil 污染的农业土壤中分离到一株能够降解 cyprodinil 的革兰氏阴性菌。16S rRNA 测序分析表明，该菌株与不动杆菌属（Acinetobacter）具有 99%的同源性。该菌株在中性条件下能有效降解 cyprodinil。在最小培养基中初始浓度为 10、20、50、100、150 和 200mg/L 时，cyprodinil 在 14 天内分别降解了 10、20、49.3、64.2、57 和 24mg/L。使用高效液相色谱串联四极杆飞行时间质谱（HPLC-QTOF-MS/MS）鉴定出两种代谢产物（4-环丙基-6-甲基-2-嘧啶吡啶胺和苯环单羟基取代物）。根据鉴定出的代谢产物，提出了一种涉及亚胺水解和苯环单羟基取代的生物降解途径。不动杆菌属（Acinetobacter）在受 cyprodinil 污染土壤的生物修复中具有潜在的应用价值，该菌株可能对嘧啶类似物的解毒和生物修复具有重要意义。
