Department of Microbiology, Bose Institute, P-1/12 C.I.T. Scheme VII M, Kolkata 700054, India.
Res Microbiol. 2013 Feb-Mar;164(2):155-63. doi: 10.1016/j.resmic.2012.11.003. Epub 2012 Nov 23.
Utilization of an enrichment technique led to isolation of a bacterium from municipal waste-contaminated soil in which acenaphthene was used as the sole source of carbon and energy. The isolate was identified as Acinetobacter sp. strain AGAT-W based on morphological, nutritional and biochemical characteristics and 16S rRNA sequence analysis. Characterization of metabolites by HPLC and GC-MS suggested hydroxylation of acenaphthene to 1-acenaphthenol, which was subsequently transformed to catechol via acenaphthenequinone, naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid and salicylic acid before entering into the tricarboxylic acid cycle. Detection of key enzymes, viz., 1-acenaphthenol dehydrogenase, salicylaldehyde dehydrogenase and catechol 1,2-dioxygenase, in the cell-free extract of Acinetobacter sp. further supported the proposed degradation pathway. This study proposes a metabolic pathway involved in acenaphthene assimilation in strain AGAT-W.
利用富集技术，从含有苊作为唯一碳源和能源的城市垃圾污染土壤中分离出一种细菌。根据形态学、营养和生化特性以及 16S rRNA 序列分析，将分离株鉴定为不动杆菌属 AGAT-W 菌株。通过 HPLC 和 GC-MS 对代谢产物进行表征，表明苊发生了羟化反应生成 1-苊酚，随后通过苊醌、萘-1,8-二羧酸、1-萘酸和水杨酸转化为儿茶酚，然后进入三羧酸循环。在不动杆菌属细胞提取物中检测到关键酶，即 1-苊酚脱氢酶、水杨醛脱氢酶和儿茶酚 1,2-双加氧酶，进一步支持了所提出的降解途径。本研究提出了 AGAT-W 菌株中参与苊同化的代谢途径。
