改良 Leeming-Notman 培养基在评估氟康唑对糠秕马拉色菌 ATCC 14521 的体外活性的 Resazurin 微量滴定法中的功效。
Efficacy of modified Leeming-Notman media in a resazurin microtiter assay in the evaluation of in-vitro activity of fluconazole against Malassezia furfur ATCC 14521.
机构信息
Department of Biomedical Science, Faculty of Medicine & Health Sciences, Universiti Putra, 43400 UPM Serdang, Selangor, Malaysia.
Department of Biomedical Science, Faculty of Medicine & Health Sciences, Universiti Putra, 43400 UPM Serdang, Selangor, Malaysia; Halal Products Research Institute, Universiti Putra, 43400 UPM Serdang, Selangor, Malaysia.
出版信息
J Mycol Med. 2018 Sep;28(3):486-491. doi: 10.1016/j.mycmed.2018.04.007. Epub 2018 May 10.
BACKGROUND
Malassezia furfur is lipodependent yeast like fungus that causes superficial mycoses such as pityriasis versicolor and dandruff. Nevertheless, there are no standard reference methods to perform susceptibility test of Malassezia species yet.
AIMS
Therefore, in this study, we evaluated the optimized culture medium for growth of this lipophilic yeast using modified leeming-Notman agar and colorimetric resazurin microtiter assay to assess antimycotic activity of fluconazole against M. furfur.
RESULTS
The result showed that these assays were more adjustable for M. furfur with reliable and reproducible MIC end-point, by confirming antimycotic activity of fluconazole with MIC of 2μg/ml.
CONCLUSION
We conclude that this method is considered as the rapid and effective susceptibility testing of M. furfur with fluconazole antifungal activity.
背景
糠秕马拉色菌是一种脂依赖性酵母样真菌，可引起浅部真菌病，如花斑癣和头皮屑。然而，目前还没有标准的参考方法来进行马拉色菌属的药敏试验。
目的
因此，在这项研究中，我们使用改良的利曼-诺特曼琼脂和比色法-resazurin 微量滴定法来评估优化的培养基，以评估氟康唑对糠秕马拉色菌的抗真菌活性。
结果
结果表明，这些方法对 M. furfur 更具可调节性，具有可靠和可重复的 MIC 终点，通过确认氟康唑的 MIC 为 2μg/ml 来证实其抗真菌活性。
结论
我们得出结论，这种方法被认为是一种快速有效的糠秕马拉色菌对氟康唑抗真菌活性的药敏试验方法。
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