Schmidt A, Rühl-Hörster B
Bayer AG, Pharma-Research - Antiinfectives, Wuppertal, Germany.
Arzneimittelforschung. 1996 Apr;46(4):442-4.
Malassezia (M.) furfur is an anthropophilic fungus with complex growth requirements. Apart from its physiological appearance on human skin it is the causative agent of several skin disorders. A method for in vitro antifungal susceptibility testing of M. furfur in a microtiter plate assay has been developed. Read-out was performed colorimetrically in modified Leeming-Notman medium after incubation with alamarBlue. Twenty-two strains of M. furfur were tested, minimum inhibitory concentrations (MICs) were determined for climbazole, piroctone-olamine, selenium disulfide, zinc pyrithione. These substances are of common use in topical therapy of M. furfur-associated skin conditions. For climbazole, the range of MICs was between < 0.03 and 2 micrograms/ml with an empirical median mean of 0.03 micrograms/ml. For piroctoneolamine the range of MICs was between 16 and 64 micrograms/ml (mean = 64 micrograms/ml), for selenium disulfide between 2 and 64 micrograms/ml (mean = 8 micrograms/ml), and for zinc pyrithione between 0.12 and 8 micrograms/ml (mean = 1 micrograms/ml). These data indicate the high in vitro activity of climbazole against M. furfur, followed by zinc pyrithione. Selenium disulfide and piroctone-olamine were less active.
糠秕马拉色菌是一种嗜人真菌，生长需求复杂。除了在人体皮肤上的生理表现外，它还是多种皮肤疾病的病原体。已开发出一种在微量滴定板试验中对糠秕马拉色菌进行体外抗真菌药敏试验的方法。在与alamarBlue孵育后，在改良的Leeming-Notman培养基中进行比色读数。测试了22株糠秕马拉色菌，测定了克霉唑、吡罗克酮乙醇胺、二硫化硒、吡啶硫酮锌的最低抑菌浓度（MIC）。这些物质常用于治疗与糠秕马拉色菌相关的皮肤疾病。对于克霉唑，MIC范围在<0.03至2微克/毫升之间，经验中位数平均值为0.03微克/毫升。对于吡罗克酮乙醇胺，MIC范围在16至64微克/毫升之间（平均值=64微克/毫升），对于二硫化硒在2至64微克/毫升之间（平均值=8微克/毫升），对于吡啶硫酮锌在0.12至8微克/毫升之间（平均值=1微克/毫升）。这些数据表明克霉唑对糠秕马拉色菌具有较高的体外活性，其次是吡啶硫酮锌。二硫化硒和吡罗克酮乙醇胺的活性较低。
