具有植物染色体的人类细胞系的产生。

Production of a Human Cell Line with a Plant Chromosome.

作者信息

Wada Naoki, Kazuki Yasuhiro, Kazuki Kanako, Inoue Toshiaki, Fukui Kiichi, Oshimura Mitsuo

机构信息

Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, 683-8503, Tottori, Japan.

Graduate School of Technology, Industrial and Social Sciences, Tokushima University, 770-8503, Tokushima, Japan.

出版信息

Methods Mol Biol. 2018;1772:289-296. doi: 10.1007/978-1-4939-7795-6_16.

DOI:10.1007/978-1-4939-7795-6_16

PMID:29754235

Abstract

It is a major challenge in biology to know whether chromosome functions of replication, segregation, gene expression, inheritance, etc. are conserved among evolutionary distant organisms where common structural features are maintained. Establishment of hybrid cell lines between evolutionary distant organisms, such as humans and plants, would be one of the promising synthetic approaches to study the evolutionary conservation of chromosome functions. In this chapter, we describe the protocol for successful establishment of human cell lines with a functional plant chromosome. Systematic analyses of hybrid cells will facilitate the evolutionary study of organisms with respect to chromosome functions. It will also provide a basic platform for genome writing and construction of chromosomal shuttle vectors .

摘要

在生物学中，一个主要挑战是要弄清楚复制、分离、基因表达、遗传等染色体功能在具有共同结构特征但进化距离较远的生物体之间是否保守。在进化距离较远的生物体（如人类和植物）之间建立杂交细胞系，将是研究染色体功能进化保守性的一种有前景的合成方法。在本章中，我们描述了成功建立具有功能性植物染色体的人类细胞系的方案。对杂交细胞进行系统分析将有助于从染色体功能方面对生物体进行进化研究。它还将为基因组编写和构建染色体穿梭载体提供一个基础平台。