基于铜离子介导的 4-巯基苯甲酸修饰的金纳米粒子，对细菌中的鸟苷三磷酸二酯 3′-二磷酸-5′-二（三）磷酸（ppGpp）进行高灵敏度和可视化检测。
Highly sensitive and visual detection of guanosine 3'-diphosphate-5'-di(tri)phosphate (ppGpp) in bacteria based on copper ions-mediated 4-mercaptobenzoic acid modified gold nanoparticles.
机构信息
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, 100039, China.
Laboratory on Pollution Monitoring and Control, College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China.
出版信息
Anal Chim Acta. 2018 Sep 6;1023:89-95. doi: 10.1016/j.aca.2018.02.082. Epub 2018 Mar 20.
Guanosine 3'-diphosphate-5'-di(tri)phosphate (ppGpp) plays a crucial role in the gene expression, metabolism, growth, and other significant processes of microorganisms. In this work, a facile sensitive and visual strategy for the detection of ppGpp has been established by developing a colorimetric probe of copper ions (Cu)-mediated 4-mercaptobenzoic acid (4-MBA) modified gold nanoparticles (AuNPs). The sensing process was characterized by transmission electron microscopy (TEM), zeta potential, dynamic light scattering (DLS) and UV-vis spectroscopy. The strategy not only achieves desirable performance over a wide concentration range (0.05-10 μM), but also exhibits excellent selectivity over other nucleotides and biomolecules. In addition, the results could be visualized by the naked eye. We have demonstrated the determination of ppGpp in Bacillus subtilis lysate samples.
鸟苷酸 3'-二磷酸-5'-二（三）磷酸（ppGpp）在微生物的基因表达、代谢、生长和其他重要过程中发挥着关键作用。在这项工作中，通过开发一种铜离子（Cu）介导的 4-巯基苯甲酸（4-MBA）修饰的金纳米粒子（AuNPs）的比色探针，建立了一种简便、灵敏和可视化的 ppGpp 检测策略。通过透射电子显微镜（TEM）、zeta 电位、动态光散射（DLS）和紫外-可见光谱对传感过程进行了表征。该策略不仅在较宽的浓度范围内（0.05-10 μM）具有理想的性能，而且对其他核苷酸和生物分子具有优异的选择性。此外，结果可以通过肉眼观察到。我们已经证明了该方法可以用于枯草芽孢杆菌裂解液样品中 ppGpp 的测定。
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