Kuo Li-Yaung, Tang Te-Yen, Li Fay-Wei, Su Huei-Jiun, Chiou Wen-Liang, Huang Yao-Moan, Wang Chun-Neng
Institute of Ecology and Evolutionary Biology, National Taiwan University, Taipei, Taiwan.
Taiwan Forestry Research Institute, Taipei, Taiwan.
Front Plant Sci. 2018 Apr 13;9:486. doi: 10.3389/fpls.2018.00486. eCollection 2018.
Organelle genomes of land plants are predominately inherited maternally but in some cases can also be transmitted paternally or biparentally. Compared to seed plants (>83% genera of angiosperms and >12% genera of gymnosperms), plastid genome (plastome) inheritance has only been investigated in fewer than 2% of fern genera, and mitochondrial genome (mitogenome) from only one fern genus. We developed a new and efficient method to examine plastome and mitogenome inheritance in a fern species- (Athyriaceae, Aspleniineae, Polypodiales), and found that plastid and mitochondrial DNAs were transmitted from only the maternal parentage to a next generation. To further examine whether both organelle genomes have the same manner of inheritance in other ferns, we sequenced both plastid and mitochondrial DNA regions of inter-species hybrids, and performed phylogenetic analyses to identify the origins of organellar DNA. Evidence from our experiments and phylogenetic analyses support that both organelle genomes in are uniparentally and maternally inherited. Most importantly, our study provides the first report of mitogenome inheritance in eupolypod ferns, and the second one among all ferns.
陆地植物的细胞器基因组主要通过母系遗传，但在某些情况下也可以通过父系或双亲遗传。与种子植物（被子植物超过83%的属和裸子植物超过12%的属）相比，质体基因组（质体基因组）的遗传仅在不到2%的蕨类植物属中进行过研究，而线粒体基因组（线粒体基因组）仅在一个蕨类植物属中进行过研究。我们开发了一种新的高效方法来检测一种蕨类植物（蹄盖蕨科、铁角蕨亚科、水龙骨目）的质体基因组和线粒体基因组遗传，发现质体和线粒体DNA仅从母本传递给下一代。为了进一步研究在其他蕨类植物中这两个细胞器基因组是否具有相同的遗传方式，我们对种间杂种的质体和线粒体DNA区域进行了测序，并进行了系统发育分析以确定细胞器DNA的来源。我们的实验和系统发育分析证据支持，这两个细胞器基因组都是单亲母系遗传的。最重要的是，我们的研究首次报道了真水龙骨蕨类植物中线粒体基因组的遗传，也是所有蕨类植物中的第二篇报道。
