Agronomic Mediterranean Institute of Bari (CIHEAM IAM Bari), Valenzano, Italy.
Dipartimento di Scienze Agrarie e Forestale (DAFNE), Universitá degli Studi della Tuscia di Viterbo, Viterbo, Italy.
J Sci Food Agric. 2018 Dec;98(15):5785-5794. doi: 10.1002/jsfa.9128. Epub 2018 Jun 25.
Ochratoxin A (OTA) is a mycotoxin produced by several species of Aspergillus and Penicillium fungi. The presence of OTA in raisins is mainly related to black Aspergillus spp. contamination. This toxin poses risks to human and animal health due to its high toxicity and carcinogenicity. New strategies to avoid the risk associated with OTA are therefore necessary.
A comparison was made between the effects of the antifungal protein PgAFP and potassium sorbate (E-202) on the growth of Aspergillus carbonarius, biosynthetic- and stress-related gene expression and its OTA production at two water activity (a ) levels, 0.95 and 0.93 a . The results showed that PgAFP successfully controlled OTA production, whereas E-202, despite being able to reduce Aspergillus carbonarius growth, caused a significant increase in OTA production by the fungus.
PgAFP protein, a biological compound with an antifungal activity, is safer to use than E-202 and may be proposed as a food preservative and a useful strategy to control ochratoxigenic A. carbonarius in raisins. © 2018 Society of Chemical Industry.
赭曲霉毒素 A（OTA）是由几种曲霉属和青霉属真菌产生的一种真菌毒素。葡萄干中 OTA 的存在主要与黑曲霉属的污染有关。由于其高毒性和致癌性，该毒素对人类和动物健康构成威胁。因此，有必要寻找新的策略来避免与 OTA 相关的风险。
比较了抗真菌蛋白 PgAFP 和山梨酸钾（E-202）对黑曲霉生长、生物合成和应激相关基因表达及其在两个水分活度（0.95 和 0.93）下产生 OTA 的影响。结果表明，PgAFP 成功地控制了 OTA 的产生，而 E-202 虽然能够抑制黑曲霉的生长，但却导致真菌产生的 OTA 显著增加。
具有抗真菌活性的生物化合物 PgAFP 比 E-202 更安全，可作为食品防腐剂和控制葡萄干中产毒黑曲霉的有用策略。 © 2018 英国化学学会。
