1 Department of Ophthalmology, St.Vincent's Hospital, College of Medicine, The Catholic University of Korea , Suwon, Korea.
2 Research Institute of Medical Science, St.Vincent's Hospital, The Catholic University of Korea , Suwon, Korea.
J Ocul Pharmacol Ther. 2018 Jun;34(5):403-409. doi: 10.1089/jop.2017.0119. Epub 2018 May 14.
To investigate the anti(lymph)angiogenic and anti-inflammatory effects of 0.5% timolol maleate in a murine corneal suture model.
Corneal neovascularization and lymphangiogenesis were compared in groups of mice that underwent corneal suture and were subsequently administered a subconjunctival injection of 0.5% timolol maleate, dexamethasone, or phosphate-buffered saline (PBS). Immunohistochemical staining and analysis were performed in each group. Real-time polymerase chain reaction (RT-PCR) was performed to quantify the expression of inflammatory cytokines [TNF-alpha and interleukin (IL)-6], vascular endothelial growth factor (VEGF)-A, VEGF-C, vascular endothelial growth factor receptor (VEGFR)-2, and VEGFR-3.
When corneas from the timolol-treated group were compared to the PBS-treated group, we observed decreases in angiogenesis, lymphangiogenesis, and inflammatory infiltration in the timolol-treated group (P value <0.05 in all respective comparisons). Corneas from the timolol-treated group showed reduced expression of VEGF-A, VEGF-C, TNF-alpha, IL-6, VEGFR-2, and VEGFR-3 compared to corneas from the PBS group (P value <0.05 in all respective comparisons).
Blocking adrenergic signaling in the cornea with 0.5% timolol maleate decreased corneal neovascularization and lymphangiogenesis.
研究马来酸噻吗洛尔 0.5%在小鼠角膜缝合模型中的抗（淋巴）血管生成和抗炎作用。
对行角膜缝合术的小鼠进行分组，分别给予结膜下注射 0.5%马来酸噻吗洛尔、地塞米松或磷酸盐缓冲盐水（PBS），比较各组角膜新生血管和淋巴管生成情况。对各组进行免疫组织化学染色和分析。采用实时聚合酶链反应（RT-PCR）检测炎症细胞因子[TNF-α和白细胞介素（IL）-6]、血管内皮生长因子（VEGF）-A、VEGF-C、血管内皮生长因子受体（VEGFR）-2 和 VEGFR-3 的表达。
与 PBS 处理组相比，马来酸噻吗洛尔处理组的角膜中血管生成、淋巴管生成和炎症浸润减少（在所有相应比较中 P 值均<0.05）。与 PBS 组相比，马来酸噻吗洛尔处理组的角膜中 VEGF-A、VEGF-C、TNF-α、IL-6、VEGFR-2 和 VEGFR-3 的表达减少（在所有相应比较中 P 值均<0.05）。
用 0.5%马来酸噻吗洛尔阻断角膜中的肾上腺素能信号可减少角膜新生血管和淋巴管生成。
Zotero 插件