Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Korea.
Molecules. 2018 May 14;23(5):1173. doi: 10.3390/molecules23051173.
(Pall.) Kuntze (TTK) is a medicinal plant traditionally used to treat various diseases such as diabetic, inflammatory, and female-related disorders. Polycystic ovary syndrome (PCOS) is a common endocrinological disorder in women of reproductive age, and hyperandrogenism is a prominent feature of PCOS resulting in anovulation and infertility. In this study, we investigated the effects of a TTK extract on androgen generation and regulation of steroidogenic enzymes in vitro and in vivo. Human adrenocortical NCI-H295R cells were used to assess the effects of TTK extract on production of dehydroepiandrosterone and testosterone, as well as the protein expression of steroidogenic enzymes. Further, a letrozole-induced PCOS rat model was used in vivo to assess whether dietary administration of TTK extract restores normal hormones and reduces PCOS symptoms. TTK extract significantly inhibited forskolin (FOR)-induced androgen production in NCI-H295R cells and serum luteinizing hormone, testosterone, and follicular cysts, but not estradiol, were reduced in letrozole-induced PCOS rats orally administered the TTK extract. In addition, TTK extract inhibits androgen biosynthesis through the ERK-CREB signaling pathway, which regulates CYP17A1 or HSD3B2 expression. TTK extract could be utilized for the prevention and treatment of hyperandrogenism and other types of PCOS.
(Pall.) 古柯 (TTK) 是一种传统的药用植物，用于治疗各种疾病，如糖尿病、炎症和女性相关疾病。多囊卵巢综合征 (PCOS) 是育龄妇女常见的内分泌紊乱，高雄激素血症是 PCOS 的一个突出特征，导致无排卵和不孕。在这项研究中，我们研究了 TTK 提取物对雄激素生成和类固醇生成酶的体外和体内调节的影响。我们使用人肾上腺皮质 NCI-H295R 细胞来评估 TTK 提取物对脱氢表雄酮和睾酮产生以及类固醇生成酶蛋白表达的影响。此外，我们还在体内使用来曲唑诱导的 PCOS 大鼠模型来评估 TTK 提取物的饮食给药是否恢复正常激素并减轻 PCOS 症状。TTK 提取物可显著抑制 NCI-H295R 细胞中 forskolin (FOR) 诱导的雄激素生成，以及血清黄体生成素、睾酮和滤泡囊肿，但对来曲唑诱导的 PCOS 大鼠的雌二醇无影响。此外，TTK 提取物通过调节 CYP17A1 或 HSD3B2 表达来抑制雄激素生物合成。TTK 提取物可用于预防和治疗高雄激素血症和其他类型的 PCOS。
