1CAS Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125,People's Republic of China.
3College of Animal Sciences and Technology,Hunan Agricultural University,Changsha 410128,People's Republic of China.
Br J Nutr. 2018 May;119(10):1119-1132. doi: 10.1017/S0007114518000727.
A total of twenty-four healthy twin-bearing Liuyang black goats were allocated to two trials. In Trial 1, twelve goats received either the control diet (CG, n 6, 100 % feed) or restricted diet (RG, n 6, 60 % feed of CG) from gestation days 26 to 65 after synchronisation. In Trial 2, the remaining goats were randomly and equally divided into two treatments: CG and RG from days 95 to 125 of gestation. Placental traits, fetal weight, serum parameters, nitric oxide (NO), angiogenesis gene expression and cotyledon proteome were measured at the end of each trial. In early pregnancy, the total and relative weights of placenta, uterine caruncle and cotyledon, as well as fetus, were increased (P<0·05) in RG. The NO content in maternal serum was also increased (P<0·05) in RG. In all, fifty differentially expressed proteins were identified in cotyledon. The up-regulated proteins are related to proliferation and fission of trophoblast cell and the placenta angiogenesis. During the late pregnancy trial, placental weight was increased (P<0·05) in RG, but weight of the fetus was decreased (P<0·05). The capillary density in the cotyledon was also decreased (P<0·01). A total of fifty-eight proteins were differentially expressed in cotyledon. The up-regulated proteins in RG are related to placenta formation, blood flow regulation and embryonic development. These results indicated that feed intake restriction during gestation influenced the placental and fetal development in a stage-dependent manner. These findings have important implications for developing novel nutrient management strategies in goat production.
共有 24 只健康的双胎浏阳黑山羊被分配到两个试验中。在试验 1 中，12 只山羊从同步后的妊娠第 26 天到第 65 天分别接受对照日粮（CG，n=6，CG 的 100%饲料）或限制日粮（RG，n=6，CG 的 60%饲料）。在试验 2 中，其余山羊随机均分为两个处理组：CG 和 RG，从妊娠第 95 天到第 125 天。每个试验结束时测量胎盘特征、胎儿重量、血清参数、一氧化氮（NO）、血管生成基因表达和胎盘中的蛋白质组。在妊娠早期，RG 组胎盘、子宫肉阜和胎盘中的总重量和相对重量以及胎儿均增加（P<0.05）。血清中 NO 含量也增加（P<0.05）。总共在胎盘中鉴定出 50 种差异表达的蛋白质。上调的蛋白质与滋养层细胞的增殖和分裂以及胎盘血管生成有关。在妊娠晚期试验中，RG 组胎盘重量增加（P<0.05），但胎儿重量减少（P<0.05）。胎盘中的毛细血管密度也降低（P<0.01）。胎盘中共有 58 种蛋白质差异表达。上调的蛋白质与胎盘形成、血流调节和胚胎发育有关。这些结果表明，妊娠期间的采食量限制以阶段依赖的方式影响胎盘和胎儿的发育。这些发现对开发山羊生产中的新型营养管理策略具有重要意义。
