孕期母体采食量限制对胎山羊生长调控、印记及表观遗传转录相关基因表达的影响
Effects of maternal feed intake restriction during pregnancy on the expression of growth regulation, imprinting and epigenetic transcription-related genes in foetal goats.
作者信息
Li Xiaopeng, Yan Qiongxian, Tang Shaoxun, Tan Zhiliang, Fitzsimmons Carolyn Jean, Yi Kangle
机构信息
CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center of Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center of Healthy Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, PR China; Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, PR China.
出版信息
Anim Reprod Sci. 2018 Nov;198:90-98. doi: 10.1016/j.anireprosci.2018.09.005. Epub 2018 Sep 6.
Maternal nutrition during gestation is a leading factor of modifying the foetal epigenome and phenotype for mammals. Imprinting genes have important roles in regulating foetal growth, programming and development. There, however, are limited data available on the effects of feed intake restriction on the expression of imprinting genes in pregnant goats. The present study, therefore, was conducted to assess the effects of maternal feed intake restriction on the relative abundance of mRNA for growth imprinting, DNA methyltransferase (DNMT) and epigenetic transcription-related genes in the liver and heart of foetal goats during gestation. A total of 24 Liuyang black goats (2.0±0.3 yr) with similar body weight (BW, 31.22±8.09 kg) and parity (2) were allocated equally to either a control group (CG) or a restriction group (RG) during both early (from 26 to 65 days) and late (from 96 to 135 days) gestation. All goats were fed a mixed diet and had free access to fresh water. The feed of the RG was 40% less than that of the CG. The early and late gestation goats were weighed, bled and slaughtered on days 65 and 135 of gestation, respectively. In early gestation, the foetal weight, body length, the weight of foetal heart and liver were greater (P < 0.05) in the RG. The CpG methylation of genomic DNA in the foetal heart was less (P = 0.0001) in the RG. The relative abundance of mRNA of methyl-CpG-binding domain protein 2 (MBD2) and methyl-CpG-binding domain protein 3 (MBD3) genes in the foetal liver were greater (P < 0.05) in the RG. During the late gestation, the foetal weight, heart weight and liver weight were less (P < 0.05) in the RG. The relative abundance of mRNA for the MBD2 gene (P = 0.043) in the foetal heart, and the ten-eleven translocation protein 1 (TET1) gene (P < 0.05) in both the foetal heart and liver were greater in the RG. These results indicate feed intake restriction during gestation influenced foetal development and regulated the relative abundance of mRNA for epigenetic transcription-related genes.
妊娠期母体营养是影响哺乳动物胎儿表观基因组和表型的主要因素。印记基因在调节胎儿生长、编程和发育方面发挥着重要作用。然而，关于采食量限制对妊娠山羊印记基因表达影响的数据有限。因此，本研究旨在评估妊娠期母体采食量限制对胎儿山羊肝脏和心脏中生长印记、DNA甲基转移酶（DNMT）和表观遗传转录相关基因mRNA相对丰度的影响。将24只体重（BW，31.22±8.09 kg）和胎次（2）相近的浏阳黑山羊（2.0±0.3岁）在妊娠早期（26至65天）和晚期（96至135天）平均分为对照组（CG）和限制组（RG）。所有山羊均饲喂混合日粮，并可自由饮用淡水。RG组的采食量比CG组少40%。分别在妊娠第65天和第135天对妊娠早期和晚期的山羊进行称重、采血和屠宰。在妊娠早期，RG组的胎儿体重、体长、胎儿心脏和肝脏重量更大（P < 0.05）。RG组胎儿心脏基因组DNA的CpG甲基化程度更低（P = 0.0001）。RG组胎儿肝脏中甲基化CpG结合结构域蛋白2（MBD2）和甲基化CpG结合结构域蛋白3（MBD3）基因的mRNA相对丰度更高（P < 0.05）。在妊娠晚期，RG组的胎儿体重、心脏重量和肝脏重量更低（P < 0.05）。RG组胎儿心脏中MBD2基因的mRNA相对丰度（P = 0.043）以及胎儿心脏和肝脏中10-11易位蛋白1（TET1）基因的mRNA相对丰度更高（P < 0.05）。这些结果表明，妊娠期采食量限制会影响胎儿发育，并调节表观遗传转录相关基因的mRNA相对丰度。
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