Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Talanta. 2019 Jul 1;199:355-360. doi: 10.1016/j.talanta.2019.02.022. Epub 2019 Feb 5.
Thiophenol is widely known as a highly toxic substance that can cause serious harm to the environment and health. Rapid and non-destructive detection of thiophenol is of great significance for environmental management. In this work, we designed and synthesized a near-infrared (NIR) fluorescent probe, (E)-4-(2-(4-(dicyanomethylene)-4H-chromen-2-yl)vinyl)-2-formylphenyl-2,4-dinitrobenzenesulfonate (DCM-CHO-D), that can respond to thiophenol rapidly (less than 3 min) based on intramolecular charge transfer (ICT) mechanism. DCM-CHO-D has high selectivity and sensitivity to the detection of thiophenol. And there is a linear relationship between the fluorescence intensity of DCM-CHO-D and thiophenol concentration in the range of 0-10 μM, the limit of detection (LOD) is as low as 0.22 μM. What's more, DCM-CHO-D can not only be used as an ideal colorimetric tool for detecting thiophenol in water samples, but also image thiophenol in living cells, indicating its potential utility for thiophenol sensing in environmental and biological samples.
噻吩酚是一种众所周知的高毒性物质，会对环境和健康造成严重危害。快速无损地检测噻吩酚对于环境管理具有重要意义。在这项工作中，我们设计并合成了一种近红外（NIR）荧光探针，（E）-4-（2-（4-（二氰基亚甲基）-2H-色烯-2-基）乙烯基）-2-甲酰基苯基-2,4-二硝基苯磺酸盐（DCM-CHO-D），它可以基于分子内电荷转移（ICT）机制快速（少于 3 分钟）响应噻吩酚。DCM-CHO-D 对噻吩酚的检测具有高选择性和灵敏度。并且 DCM-CHO-D 的荧光强度与噻吩酚浓度在 0-10 μM 范围内呈线性关系，检测限（LOD）低至 0.22 μM。更重要的是，DCM-CHO-D 不仅可以用作水样中检测噻吩酚的理想比色工具，还可以在活细胞中成像噻吩酚，表明其在环境和生物样品中检测噻吩酚的潜在用途。
