Mukherjee Chandrayana, Hale Christine, Mukhopadhyay Subhankar
Metabolic Research Laboratories, Wellcome Trust-MRC Institute of Metabolic Science, Addenbrooke's Hospital, University of Cambridge, Cambridge, UK.
The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Cambridge, UK.
Methods Mol Biol. 2018;1784:13-28. doi: 10.1007/978-1-4939-7837-3_2.
Macrophages differentiated from human induced pluripotent stem cells (hiPSCs) provide an alternative new tool overcoming some of the limitations of existing models for human macrophages, such as human macrophage-like cell lines and primary monocyte-derived macrophages. A combination of different cytokines and growth factors can differentiate hiPSCs toward myeloid lineage. Here we describe a simple multistep protocol for differentiating hiPSCs into functional macrophages. This includes derivation of three germ-line containing embryoid bodies (EBs) from iPSCs, generation of myeloid precursors from EBs, and finally maturation of myeloid precursors into functional macrophages. Technical procedure and specific culture conditions associated with each of these steps are discussed in detail.
从人诱导多能干细胞（hiPSC）分化而来的巨噬细胞提供了一种替代性新工具，克服了现有人类巨噬细胞模型（如人巨噬细胞样细胞系和原代单核细胞衍生的巨噬细胞）的一些局限性。不同细胞因子和生长因子的组合可使hiPSC向髓系分化。在此，我们描述了一种将hiPSC分化为功能性巨噬细胞的简单多步骤方案。这包括从iPSC衍生出包含三个胚层的胚状体（EB），从EB生成髓系前体细胞，最后将髓系前体细胞成熟为功能性巨噬细胞。详细讨论了与这些步骤中的每一步相关的技术程序和特定培养条件。
