Moreno Stéphanie G
CEA/DRF/IBFJ/iRCM/LRTS, Fontenay aux Roses, France.
INSERM U967, Fontenay aux Roses, France.
Methods Mol Biol. 2018;1784:259-262. doi: 10.1007/978-1-4939-7837-3_23.
In order to decipher the role of macrophages in vivo, it could be necessary to establish a model of macrophage depletion in the whole animal. One method to obtain animal models efficiently depleted in macrophages in different tissues (bone marrow, spleen, liver, lungs, brain, gut, peritoneal cavity, lymph nodes/vessels) and blood is the use of a clodronate-liposome solution.Here, we describe the protocol used to deplete efficiently macrophages in mouse bone marrow.
为了解巨噬细胞在体内的作用，可能有必要建立一种在整个动物体内使巨噬细胞耗竭的模型。一种有效获得不同组织（骨髓、脾脏、肝脏、肺、脑、肠道、腹腔、淋巴结/血管）和血液中巨噬细胞耗竭的动物模型的方法是使用氯膦酸盐脂质体溶液。在此，我们描述了用于有效耗竭小鼠骨髓中巨噬细胞的方案。
