Oussoren C, Velinova M, Scherphof G, van der Want J J, van Rooijen N, Storm G
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS ), Utrecht University, Utrecht, Netherlands.
Biochim Biophys Acta. 1998 Mar 13;1370(2):259-72. doi: 10.1016/s0005-2736(97)00275-7.
The ability of clodronate-containing liposomes to deplete lymph nodes of macrophages was used as a tool to investigate the fate of liposomes in regional lymph nodes after subcutaneous (s.c.) administration. Reduced lymph node localization of liposomes in macrophage-depleted lymph nodes confirmed that phagocytosis by macrophages plays an important role in lymph node retention of liposomes. Depletion of macrophages had less effect on lymph node localization of small liposomes than on the lymph node localization of large liposomes. Inclusion of distearoylphosphatidylethanolamine (DSPE)-poly(ethyleneglycol) (PEG-PE) into the liposomes, which is known to oppose macrophage uptake, did not affect lymph node localization in macrophage-depleted or control lymph nodes. We conclude that PEG-liposomes retained by lymph nodes are also taken up by lymph node macrophages. Morphological observations visualizing the uptake of PEG-liposomes by lymph node macrophages support this conclusion.
含氯膦酸盐脂质体清除淋巴结中巨噬细胞的能力被用作一种工具，以研究皮下给药后脂质体在区域淋巴结中的命运。在巨噬细胞耗竭的淋巴结中，脂质体在淋巴结中的定位减少，这证实巨噬细胞的吞噬作用在脂质体的淋巴结滞留中起重要作用。巨噬细胞的耗竭对小脂质体的淋巴结定位影响比对大脂质体的淋巴结定位影响小。将已知可对抗巨噬细胞摄取的二硬脂酰磷脂酰乙醇胺（DSPE）-聚乙二醇（PEG-PE）包含在脂质体中，对巨噬细胞耗竭的或对照淋巴结中的淋巴结定位没有影响。我们得出结论，被淋巴结保留的聚乙二醇脂质体也被淋巴结巨噬细胞摄取。可视化淋巴结巨噬细胞摄取聚乙二醇脂质体的形态学观察结果支持这一结论。
