一种用于应激相关植物激素靶向分析的超高效液相色谱-串联质谱法
An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones.
作者信息
Novák Ondřej, Floková Kristýna
机构信息
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic.
Faculty of Science, Palacký University, Olomouc, Czech Republic.
出版信息
Methods Mol Biol. 2018;1778:183-192. doi: 10.1007/978-1-4939-7819-9_13.
The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
本文所述方法代表了一种改进策略，用于分析多种与胁迫相关的植物激素，包括茉莉酸、水杨酸、脱落酸和生长素代谢物。优化了方法条件，以降低复杂植物基质的背景效应，实现有效的预浓缩，从而通过超高效液相色谱-串联质谱（UHPLC-MS/MS）对多种植物激素进行高灵敏度分析。
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