Orthodontic Section, Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Department of Oral Biology, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
J Periodontol. 2018 Sep;89(9):1131-1141. doi: 10.1002/JPER.17-0653. Epub 2018 Aug 14.
Although vibration combined with orthodontic force may accelerate orthodontic tooth movement, little is known about the mechanisms that regulate the associated cellular responses. The goal of this study was to investigate whether mechanical vibration combined with compressive force regulates cyclooxygenase (COX)-2/prostaglandin E2 (PGE ) and interleukin (IL)-6 and IL-8 messenger RNA (mRNA) and protein expression in human periodontal ligament (hPDL) cells via the COX pathway.
The primary cultured hPDL cells were exposed to mechanical vibration, compressive force or a combination of both mechanical vibration and compressive force at 24, 48, and 72 hours. The COX-2, IL-6, IL-8, receptor activator of nuclear factor kappa-Β ligand (RANKL), and osteoprotegrin (OPG) mRNA expressions were determined using quantitative real-time polymerase chain reaction (qPCR). The PGE , IL-6, and IL-8 protein expressions were quantified by enzyme-linked immunosorbent assay (ELISA). To demonstrate whether the expression of PGE , IL-6, and IL-8 was in the COX-dependent pathway, the hPDL cells were treated with indomethacin. To determine whether PGE stimulated the hPDL cells to express IL-6 and IL-8, exogenous PGE was added to the culture media.
The combination of mechanical vibration and compressive force synergistically upregulated RANKL/OPG, COX-2/PGE , IL-6 and IL-8 mRNA, and protein expression. The indomethacin significantly attenuated the increases of PGE , IL-6, and IL-8 expression in cells stimulated with compressive force or mechanical vibration combined with compressive force. In addition, exogenous PGE increased IL-6 and IL-8 mRNA and protein expressions in hPDL cells.
Mechanical vibration may enhance alveolar bone resorption at the compression side during orthodontic tooth movement via a mechanism involving the cyclooxygenase pathway.
虽然振动联合正畸力可能会加速牙齿正畸移动，但对于调节相关细胞反应的机制知之甚少。本研究旨在探讨机械振动联合压力是否通过环氧化酶（COX）途径调节人牙周膜（hPDL）细胞中环氧化酶-2（COX-2）/前列腺素 E2（PGE2）和白细胞介素（IL）-6 和 IL-8 的信使 RNA（mRNA）和蛋白表达。
原代培养的 hPDL 细胞分别暴露于机械振动、压力或机械振动和压力联合作用 24、48 和 72 小时。采用实时定量聚合酶链反应（qPCR）检测 COX-2、IL-6、IL-8、核因子κB 受体激活剂配体（RANKL）和骨保护素（OPG）mRNA 的表达。酶联免疫吸附试验（ELISA）定量检测 PGE2、IL-6 和 IL-8 蛋白的表达。为了证明 PGE2、IL-6 和 IL-8 的表达是否依赖 COX 途径，用吲哚美辛处理 hPDL 细胞。为了确定 PGE2 是否刺激 hPDL 细胞表达 IL-6 和 IL-8，将外源性 PGE2 添加到培养基中。
机械振动与压力联合作用协同上调 RANKL/OPG、COX-2/PGE2、IL-6 和 IL-8 mRNA 和蛋白表达。吲哚美辛显著抑制了压力或机械振动联合压力刺激细胞中 PGE2、IL-6 和 IL-8 表达的增加。此外，外源性 PGE2 增加了 hPDL 细胞中 IL-6 和 IL-8 的 mRNA 和蛋白表达。
机械振动可能通过环氧化酶途径增强正畸牙齿移动过程中受压侧牙槽骨的吸收。
