• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从受油污污染的环境中分离出的生物表面活性剂（包括一种新的表面活性剂）的鉴定和超高效液相色谱-高分辨率质谱联用分析。

Identification and ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry characterization of biosurfactants, including a new surfactin, isolated from oil-contaminated environments.

机构信息

Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9500, 91501-970, Porto Alegre, RS, Brazil.

Departamento de Química, Universidade Estadual de Maringá (UEM), 87020-900, Maringá, PR, Brazil.

出版信息

Microb Biotechnol. 2018 Jul;11(4):759-769. doi: 10.1111/1751-7915.13276. Epub 2018 May 14.

DOI:10.1111/1751-7915.13276
PMID:29761667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6011949/
Abstract

Biosurfactant-producing bacteria were isolated from samples collected in areas contaminated with crude oil. The isolates were screened for biosurfactant production using qualitative drop-collapse test, oil-spreading and emulsification assays, and measurement of their tensoactive properties. Five isolates tested positive for in the screening experiments and displayed decrease in the surface tension below 30 mN m . The biosurfactants produced by these isolates were further investigated and their molecular identification revealed that they are bacteria related to the Bacillus genus. Additionally, the biosurfactants produced were chemically characterized via UHPLC-HRMS experiments, indicating the production of surfactin homologues, including a new class of these molecules.

摘要

从受原油污染的区域采集的样本中分离出了产生生物表面活性剂的细菌。使用定性滴溃试验、油扩散和乳化试验以及张力活性特性测量对分离物进行了生物表面活性剂产生的筛选。在筛选试验中，有 5 个分离物呈阳性，表面张力下降到 30 mN/m 以下。对这些分离物产生的生物表面活性剂进行了进一步研究，其分子鉴定表明它们是与芽孢杆菌属有关的细菌。此外，通过 UHPLC-HRMS 实验对产生的生物表面活性剂进行了化学表征，表明产生了类似表面活性剂的物质，包括这些分子的一个新类别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/2792f5328924/MBT2-11-759-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/699c36c5e8e4/MBT2-11-759-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/0244357204d6/MBT2-11-759-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/cafbda762ecb/MBT2-11-759-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/df4f19125953/MBT2-11-759-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/2792f5328924/MBT2-11-759-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/699c36c5e8e4/MBT2-11-759-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/0244357204d6/MBT2-11-759-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/cafbda762ecb/MBT2-11-759-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/df4f19125953/MBT2-11-759-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c14/6011949/2792f5328924/MBT2-11-759-g005.jpg

相似文献

1
Identification and ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry characterization of biosurfactants, including a new surfactin, isolated from oil-contaminated environments.从受油污污染的环境中分离出的生物表面活性剂（包括一种新的表面活性剂）的鉴定和超高效液相色谱-高分辨率质谱联用分析。
Microb Biotechnol. 2018 Jul;11(4):759-769. doi: 10.1111/1751-7915.13276. Epub 2018 May 14.
2
Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation.新型菌株 Bacillus nealsonii S2MT 所产表面活性剂样生物表面活性剂的生产及特性及其在油污土壤修复中的应用潜力。
Microb Cell Fact. 2020 Jul 20;19(1):145. doi: 10.1186/s12934-020-01402-4.
3
Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.萎缩芽孢杆菌5-2a产脂肽生物表面活性剂及其在微生物强化采油中的潜在应用。
Microb Cell Fact. 2016 Oct 3;15(1):168. doi: 10.1186/s12934-016-0574-8.
4
Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge.从含油污泥中提高采油率的细菌分离物——解淀粉芽孢杆菌 BZ-6 的分离、鉴定和特性研究。
Chemosphere. 2012 Jun;87(10):1105-10. doi: 10.1016/j.chemosphere.2012.01.059. Epub 2012 Feb 24.
5
Characterization and properties of biosurfactants produced by a newly isolated strain Bacillus methylotrophicus DCS1 and their applications in enhancing solubility of hydrocarbon.新分离菌株甲基营养型芽孢杆菌DCS1产生的生物表面活性剂的表征、性质及其在提高烃类溶解度中的应用
World J Microbiol Biotechnol. 2016 Nov;32(11):175. doi: 10.1007/s11274-016-2132-2. Epub 2016 Sep 15.
6
Isolation and molecular characterization of biosurfactant producing yeasts from the soil samples contaminated with petroleum derivatives.从受石油衍生物污染的土壤样本中分离和鉴定产生生物表面活性剂的酵母及其分子特征。
J Basic Microbiol. 2018 Sep;58(9):782-792. doi: 10.1002/jobm.201800126. Epub 2018 Jun 25.
7
Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil.从受柴油污染土壤中分离出的生物表面活性剂产生微生物的多样性。
Microbiol Res. 2005;160(3):249-55. doi: 10.1016/j.micres.2004.08.005.
8
[Isolation and identification of surfactin producing Bacillus subtilis strain and its effect of surfactin on crude oil].[产表面活性素枯草芽孢杆菌菌株的分离鉴定及其表面活性素对原油的作用]
Wei Sheng Wu Xue Bao. 2008 Mar;48(3):304-11.
9
Characterization of surfactin produced by Bacillus subtilis isolate BS5.枯草芽孢杆菌分离株BS5产生的表面活性素的特性分析。
Appl Biochem Biotechnol. 2008 Sep;150(3):289-303. doi: 10.1007/s12010-008-8153-z. Epub 2008 Apr 25.
10
Detection of biosurfactants in Bacillus species: genes and products identification.芽孢杆菌属中生物表面活性剂的检测：基因与产物鉴定
J Appl Microbiol. 2015 Oct;119(4):1023-34. doi: 10.1111/jam.12893. Epub 2015 Sep 4.

引用本文的文献

1
Genome mining, structural elucidation and surface-active property of a new lipopeptide from Bacillus subtilis.枯草芽孢杆菌新脂肽的基因组挖掘、结构解析及表面活性特性
Microb Cell Fact. 2025 May 14;24(1):106. doi: 10.1186/s12934-025-02723-y.
2
Exploration and characterization of a newly isolated bacterium, Enterobacter quasihormaechei strain BDIFST24001, capable of producing rhamnolipid biosurfactant for oil remediation.对新分离的一株能够产生鼠李糖脂生物表面活性剂用于石油修复的准霍氏肠杆菌BDIFST24001进行探索与特性分析。
Access Microbiol. 2024 Aug 1;6(8). doi: 10.1099/acmi.0.000830.v4. eCollection 2024.
3
Enhanced low-cost lipopeptide biosurfactant production by Bacillus velezensis from residual glycerin.

本文引用的文献

1
Carbon source effects on the mono/dirhamnolipid ratio produced by Pseudomonas aeruginosa L05, a new human respiratory isolate.碳源对新型人呼吸道分离菌铜绿假单胞菌 L05 所产单/二鼠李糖脂比例的影响。
N Biotechnol. 2017 Oct 25;39(Pt A):36-41. doi: 10.1016/j.nbt.2017.05.013. Epub 2017 Jun 3.
2
Variants of lipopeptides and glycolipids produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa cultured in different carbon substrates.在不同碳源底物中培养的解淀粉芽孢杆菌和铜绿假单胞菌产生的脂肽和糖脂变体。
AMB Express. 2017 Dec;7(1):109. doi: 10.1186/s13568-017-0367-4. Epub 2017 May 31.
3
Biosurfactant surfactin with pH-regulated emulsification activity for efficient oil separation when used as emulsifier.
来自解淀粉芽孢杆菌的强化低成本脂肽生物表面活性剂的生产，以残余甘油为原料。
Bioprocess Biosyst Eng. 2024 Sep;47(9):1555-1570. doi: 10.1007/s00449-024-03051-y. Epub 2024 Jun 25.
4
Screening Plant Growth-Promoting Bacteria with Antimicrobial Properties for Upland Rice.筛选具有抗微生物特性的促进旱地水稻生长的细菌。
J Microbiol Biotechnol. 2024 May 28;34(5):1029-1039. doi: 10.4014/jmb.2402.02008. Epub 2024 Apr 1.
5
Antibiofilm Activity of the Marine Probiotic Bacillus subtilis C3 Against the Aquaculture-Relevant Pathogen Vibrio harveyi.海洋益生菌枯草芽孢杆菌C3对水产养殖相关病原菌哈维氏弧菌的抗生物膜活性
Probiotics Antimicrob Proteins. 2025 Jun;17(3):1551-1562. doi: 10.1007/s12602-024-10229-z. Epub 2024 Feb 8.
6
Molecular characterization and evaluation of surfactins produced by endophytic bacteria from .来自……的内生细菌产生的表面活性素的分子表征与评估
Front Chem. 2023 Aug 7;11:1240704. doi: 10.3389/fchem.2023.1240704. eCollection 2023.
7
Identifications of Surfactin-Type Biosurfactants Produced by Species Isolated from Rhizosphere of Vegetables.从蔬菜根际中分离得到的 种产生的表面活性剂型生物表面活性剂的鉴定。
Molecules. 2023 Jan 25;28(3):1172. doi: 10.3390/molecules28031172.
8
A review on the physicochemical and biological applications of biosurfactants in biotechnology and pharmaceuticals.生物表面活性剂在生物技术和制药领域的物理化学及生物学应用综述。
Heliyon. 2022 Aug 8;8(8):e10149. doi: 10.1016/j.heliyon.2022.e10149. eCollection 2022 Aug.
9
Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking.利用分子网络技术对海洋细菌中的抗菌生物表面活性剂进行去重复。
Sci Rep. 2021 Aug 11;11(1):16286. doi: 10.1038/s41598-021-95788-9.
10
Sustainable Surfactin Production by Using Crude Glycerol from Different Wastes.利用不同废物中的粗甘油可持续生产表面活性剂。
Molecules. 2021 Jun 8;26(12):3488. doi: 10.3390/molecules26123488.
作为乳化剂使用时，具有 pH 调控乳化活性的生物表面活性剂表面活性剂可实现高效的油水分离。
Bioresour Technol. 2017 Oct;241:200-206. doi: 10.1016/j.biortech.2017.05.120. Epub 2017 May 20.
4
Direct quantification of lipopeptide biosurfactants in biological samples via HPLC and UPLC-MS requires sample modification with an organic solvent.通过高效液相色谱（HPLC）和超高效液相色谱-质谱联用（UPLC-MS）对生物样品中的脂肽生物表面活性剂进行直接定量分析，需要用有机溶剂对样品进行预处理。
Appl Microbiol Biotechnol. 2017 Jun;101(11):4747-4759. doi: 10.1007/s00253-017-8272-y. Epub 2017 Apr 22.
5
High-performance thin-layer chromatography (HPTLC) for the simultaneous quantification of the cyclic lipopeptides Surfactin, Iturin A and Fengycin in culture samples of Bacillus species.用于同时定量芽孢杆菌属培养样品中环脂肽表面活性素、伊枯草菌素A和丰原素的高效薄层色谱法（HPTLC）
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Feb 15;1044-1045:214-224. doi: 10.1016/j.jchromb.2016.11.013. Epub 2016 Nov 10.
6
Clarification of the Antagonistic Effect of the Lipopeptides Produced by Bacillus amyloliquefaciens BPD1 against Pyricularia oryzae via In Situ MALDI-TOF IMS Analysis.通过原位基质辅助激光解吸电离飞行时间成像质谱分析阐明解淀粉芽孢杆菌BPD1产生的脂肽对稻瘟病菌的拮抗作用
Molecules. 2016 Dec 3;21(12):1670. doi: 10.3390/molecules21121670.
7
Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking.通过全球天然产物社会分子网络共享和社区管理质谱数据。
Nat Biotechnol. 2016 Aug 9;34(8):828-837. doi: 10.1038/nbt.3597.
8
A novel high-throughput and quantitative method based on visible color shifts for screening Bacillus subtilis THY-15 for surfactin production.一种基于可见颜色变化的新型高通量定量方法，用于筛选枯草芽孢杆菌THY-15以生产表面活性素。
J Ind Microbiol Biotechnol. 2015 Aug;42(8):1139-47. doi: 10.1007/s10295-015-1635-4. Epub 2015 Jun 12.
9
Biosurfactant production by Pseudomonas strains isolated from floral nectar.从花蜜中分离出的假单胞菌菌株产生生物表面活性剂。
J Appl Microbiol. 2015 Jun;118(6):1370-84. doi: 10.1111/jam.12799. Epub 2015 Apr 22.
10
Lipopeptides as the antifungal and antibacterial agents: applications in food safety and therapeutics.脂肽作为抗真菌和抗菌剂：在食品安全与治疗中的应用。
Biomed Res Int. 2015;2015:473050. doi: 10.1155/2015/473050. Epub 2015 Jan 6.