The National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou 450002, China.
The Collaborative Center Innovation of Henan Food Crops, Henan Agricultural University, Zhengzhou 450002, China.
Int J Mol Sci. 2018 May 15;19(5):1465. doi: 10.3390/ijms19051465.
The APETALA2/ethylene response factor (AP2/ERF) superfamily is involved in the responses of plants to biotic and abiotic stresses; however, the functions and mechanisms of some members of this family in plants are unclear. In our previous study, expression of , a member of the AP2/ERF family, was remarkably induced in wheat seedlings suffering freezing stress. In this study, we show that its expression was rapidly upregulated in response to salt, cold, and water deficiency, suggesting roles in the responses to abiotic stresses. Further, transient barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) resulted in significantly reduced tolerance to 20% PEG6000-stimulated water deficiency. Subcellular localization and transcriptional activation assays separately showed that TaERFL1a was targeted to the nucleus and possessed transcriptional activation activity. Yeast two-hybrid library screening identified six interacting proteins, and of these, the interactions between TaERFL1a and TaSGT1, and TaERFL1a and TaDAD2 proteins were further confirmed by yeast co-transformation and bimolecular fluorescent complementation (BiFC). Collectively, our results suggest that TaERFL1a is a stress-responsive transcription factor, which could be functionally related to proteins involved in the abiotic stress responses of plants.
AP2/ERF 超家族参与植物对生物和非生物胁迫的响应；然而，该家族的一些成员在植物中的功能和机制尚不清楚。在我们之前的研究中，AP2/ERF 家族的一个成员 的表达在遭受冷冻胁迫的小麦幼苗中显著诱导。在这项研究中，我们表明其表达对盐、冷和水分缺乏迅速上调，表明其在非生物胁迫响应中的作用。此外，瞬时大麦条纹花叶病毒诱导的基因沉默（BSMV-VIGS）导致对 20%PEG6000 刺激的水分缺乏的耐受性显著降低。亚细胞定位和转录激活测定分别表明 TaERFL1a 靶向细胞核并具有转录激活活性。酵母双杂交文库筛选鉴定出六个相互作用蛋白，其中 TaERFL1a 与 TaSGT1 和 TaERFL1a 与 TaDAD2 蛋白之间的相互作用通过酵母共转化和双分子荧光互补（BiFC）进一步证实。总之，我们的结果表明 TaERFL1a 是一种应激响应转录因子，可能与参与植物非生物胁迫响应的蛋白质在功能上相关。
