School of Clinical Medicine, Tsinghua University, China.
Eur Rev Med Pharmacol Sci. 2018 Apr;22(8):2343-2350. doi: 10.26355/eurrev_201804_14825.
Glioma is a tumor of the brain. Although the clinical regimens and surgical techniques for glioma have improved, therapies of advanced glioma remain challenging, carrying dismal overall survival and therapeutic success rates. Evidence has shown that miRNAs played important roles in glioma development. The current study aimed at investigating the function of a novel cancerogenic miRNA, miR-93, in glioma progression by investigating the expression and mechanism of it.
qRT-PCR was conducted to assess the miR-93 expression and the mRNA expression of target gene in glioma tissues and cells. The invasion and migration abilities of the glioma cells were determined by transwell assays. Luciferase reporter assay was performed to confirm the target of miR-93.
The results indicated that miR-93 expression in glioma tissues and cells was increased significantly than that in normal brain tissues and cells. Furthermore, miR-93 promoted glioma cell migration and invasion. RBL2 was recognized as a direct target of miR-93 in glioma cells, and overexpression of RBL2 could reverse the stimulative effect of miR-93 in glioma cell.
The above findings suggested that miR-93 together with RBL2 could be diagnostic targets and novel prognostic markers for glioma.
脑胶质瘤是一种脑肿瘤。尽管脑胶质瘤的临床治疗方案和手术技术有所提高，但高级别脑胶质瘤的治疗仍然具有挑战性，总体生存率和治疗成功率都很低。有证据表明，miRNA 在脑胶质瘤的发生发展中发挥着重要作用。本研究旨在通过研究其表达和机制，探讨新型致癌 miRNA miR-93 在脑胶质瘤进展中的作用。
采用 qRT-PCR 检测脑胶质瘤组织和细胞中 miR-93 的表达以及靶基因的 mRNA 表达。通过 Transwell 实验测定脑胶质瘤细胞的侵袭和迁移能力。通过荧光素酶报告实验证实 miR-93 的靶基因。
结果表明，miR-93 在脑胶质瘤组织和细胞中的表达明显高于正常脑组织和细胞。此外，miR-93 促进了脑胶质瘤细胞的迁移和侵袭。RBL2 被认为是脑胶质瘤细胞中 miR-93 的直接靶基因，过表达 RBL2 可以逆转 miR-93 对脑胶质瘤细胞的刺激作用。
上述研究结果表明，miR-93 与 RBL2 一起可以作为脑胶质瘤的诊断靶点和新的预后标志物。
